We narrowed to 57,378 results for: plasmid
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Plasmid#21220DepositorInsertCAMKIIalpha-T286A (Camk2a Rat)
TagsGFPExpressionMammalianMutationT286A (Lab plasmid KS 0003).Available SinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-eGFP
Plasmid#62518PurposeExpresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.DepositorInserteGFP
UseAAVExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
Tagstriplicated NLS sequence DPKKKRKVExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-RiboL1-jGCaMP8s
Plasmid#192616PurposeAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.DepositorInsertFLEX-RIboL1-jGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-ER-TurboID
Plasmid#149412PurposeMuscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5PromotertMCKAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD171
Plasmid#229483PurposeAdenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genesDepositorInsertE2A, VA RNA I, L4-22k/33k
UseAAV and AdenoviralExpressionMammalianPromoterCMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxExpressionMammalianPromoterEf1a, Human elongation factor-1 alphaAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-AAVS1
Plasmid#227272PurposeExpresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.DepositorAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV-PHP.eB-CMV-eGFP
Plasmid#230929PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVPromoterCMVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-28:TUBA1B-mTagRFP-T
Plasmid#101785PurposeHomology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ERK-KTR-mStrawberry reporter
Plasmid#158686PurposeThis plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161927PurposeDonor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine repression reporter
UseSynthetic BiologyExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPP537
Plasmid#158803PurposepRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
Tags2xSV40NLS-6xHisExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
spisPink chromoprotein
Plasmid#117839PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coliDepositorInsertpromoter, RBS, spisPink
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AK1-His
Plasmid#124135PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymScarletI-CaURA3
Plasmid#118457PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.DepositorInsertymScarletI
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only