Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 6,374 results for: Mag

Showing: 5461 - 5490 of 6374 results

  1. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118722-plasmid-map-sequence-id-230466

    Enlarge

  2. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118720-plasmid-map-sequence-id-230457

    Enlarge

  3. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

    Enlarge

  5. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid
    #196223
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196223-plasmid-map-sequence-id-405486

    Enlarge

  6. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Expression
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

    Enlarge

  7. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

    Enlarge

  8. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

    Plasmid
    #196224
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196224-plasmid-map-sequence-id-405478

    Enlarge

  9. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

    Enlarge

  10. 18065-M01-412

    Plasmid
    #225664
    Purpose
    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue
    Depositor
    Dominic Esposito
    Article
    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:
    Inserts
    dsCopGFP
    delCD19 (CD19 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    C-terminally truncated (aa 1-333 only)
    Promoter
    SORE6-mCMVp and WPRE-SV40p
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225664-plasmid-map-sequence-id-452346

    Enlarge

  11. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid
    #197507
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197507-plasmid-map-sequence-id-386698

    Enlarge

  12. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

    Enlarge

  14. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

    Enlarge

  15. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid
    #175181
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175181-plasmid-map-sequence-id-345963

    Enlarge

  16. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

    Enlarge

  17. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

    Plasmid
    #104495
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7s
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    104495-plasmid-map-sequence-id-200303

    Enlarge

  20. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR1 (IFNGR1 Human)
    Use
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192786-plasmid-map-sequence-id-379664

    Enlarge

  22. pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE

    Plasmid
    #105322
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV1
    Insert
    jGCaMP7c variant 1513
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    105322-plasmid-map-sequence-id-213536

    Enlarge

  24. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid
    #175184
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175184-plasmid-map-sequence-id-345962

    Enlarge

  26. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt

    Plasmid
    #175180
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    175180-plasmid-map-sequence-id-343768

    Enlarge

  28. pT2-shATRX-GFP

    Plasmid
    #124259
    Purpose
    Expresses shRNA targeting ATRX with a GFP reporter which is driven by the SV40 promoter. Construct has inverted repeats to be used in Sleeping beauty system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    shATRX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124259-plasmid-map-sequence-id-248702

    Enlarge

  29. pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt

    Plasmid
    #175182
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    175182-plasmid-map-sequence-id-343767

    Enlarge

Showing: 5461 - 5490 of 6374 results