We narrowed to 10,182 results for: yeast
-
Plasmid#198665PurposeAn Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiaeDepositorInsertX3_UP
UseSynthetic BiologyExpressionYeastAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.F02R
Plasmid#198662PurposeEasy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding endsDepositorInsertlinker+GFP
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.F02L
Plasmid#198661PurposeEasy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with CD protruding endsDepositorInsertlinker+GFP
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.T02R
Plasmid#198654PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding endsDepositorInserttCYC1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.T01L
Plasmid#198653PurposeEasy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding endsDepositorInserttADH1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P06R
Plasmid#198652PurposeEasy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding endsDepositorInsertpPDA1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P06L
Plasmid#198651PurposeEasy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding endsDepositorInsertpPDA1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P05R
Plasmid#198650PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding endsDepositorInsertpCYC1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P05L
Plasmid#198649PurposeEasy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding endsDepositorInsertpCYC1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P04R
Plasmid#198648PurposeEasy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding endsDepositorInsertpTPI1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P04L
Plasmid#198647PurposeEasy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding endsDepositorInsertpTPI1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03R
Plasmid#198646PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding endsDepositorInsertpPGK1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P03L
Plasmid#198645PurposeEasy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding endsDepositorInsertpPGK1
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02R
Plasmid#198644PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding endsDepositorInsertpENO2
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P02L
Plasmid#198643PurposeEasy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding endsDepositorInsertpENO2
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01R
Plasmid#198642PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding endsDepositorInsertpTDH3
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEM.P01L
Plasmid#198641PurposeEasy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding endsDepositorInsertpTDH3
UseSynthetic BiologyExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
TagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 WT (123-204)
Plasmid#196834PurposeExpresses Pab1 RRM2 fragment fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acidsPromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 E181R (123-204)
Plasmid#196835PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acids & E181RPromoterADHAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRuby3 only VLP
Plasmid#182429PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-mRuby3
deltaVP1
UseSynthetic BiologyExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.103
Plasmid#184988PurposeExpress Cas9-P2A-Eco1RTDepositorInsertCas9-P2A-Eco1RT
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.102
Plasmid#184987PurposeExpress -Eco1 RT-P2A-Cas9DepositorInsertEco1RT-P2A-Cas9
TagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14
Plasmid#183062PurposePlasmid expressing a biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under RET2p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterHREsYGP1p and RET2Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2S
Plasmid#185865PurposeKnocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiaeDepositorInsertALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 b2
Plasmid#188425PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2DepositorInsertAuxin Signaling Receptor AFB2/3 b2
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-ZmAFB2/3 a
Plasmid#188423PurposeZea Mays Nuclear Auxin Signaling Receptor AFB2/3 ADepositorInsertAuxin Signaling Receptor AFB2/3 a
ExpressionYeastAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB441
Plasmid#185131PurposeSwi6 full length with L->A mutated in nuclear localization signalDepositorInsertSWI6
TagsGFPExpressionYeastMutationSWI6 deletion of amino acids 450-458Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
TagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB280
Plasmid#185073PurposeLarger fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111 aa 1-83Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB282
Plasmid#185074PurposeSmaller fusion of Nma111 nuclear localization signal to GFPDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111 aa 1-35Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB419
Plasmid#185089PurposeSwi6L-GFP expressing N-term 273 amino acids of Swi6DepositorInsertSWI6
TagsGFPExpressionYeastMutationSwi6 truncation at aa 273Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB465
Plasmid#185098PurposeNMA111-GFP with NLS1 deleted under endogenous promoterDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111DNLS1-GFPAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB462
Plasmid#185095PurposeNMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoterDepositorInsertNMA111
TagsGFPExpressionYeastMutationGAL1::nma111Dnls1Dnls2-GFPPromoterGAL1Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB466
Plasmid#185099PurposeNMA111-GFP with NLS1 and NLS2 deleted under endogenous promoterDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111DNLS1DNLS2-GFPAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB467
Plasmid#185100PurposeNMA111-GFP with NLS2 deleted under endogenous promoterDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111DNLS2-GFPAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB354
Plasmid#185082PurposeSwi6L-GFP expressin N-term 273 amino acids of Swi6DepositorInsertSWI6
TagsGFPExpressionYeastMutationSwi6 truncation aa 1-273Available SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB416
Plasmid#185087PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
TagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB353
Plasmid#185081PurposeSwi6M-GFP expressing N-term 181 amino acids of Swi6DepositorInsertSWI6
TagsGFPExpressionYeastMutationSwi6 truncation aa 1-181Available SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB484
Plasmid#185132PurposeSWI6-GFP full length under control of GAL1 promotionDepositorInsertSWI6
TagsGFPExpressionYeastAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB486
Plasmid#185133PurposeFull length CRM1 fused to binding domain of two-hybrid plasmidDepositorInsertCRM1
ExpressionYeastAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB491
Plasmid#185134PurposeFull length SWI6 fused to activation domain of two-hybrid plasmidDepositorInsertSWI6
ExpressionYeastAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL30
Plasmid#174064PurposeEncodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.DepositorInsertpGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
ExpressionBacterial and YeastAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Rnt1-dsRBD
Plasmid#171549PurposeEncoding part of the budding yeast Rnt1 protein from L366 to S453DepositorInsertRnt1 (RNT1 Budding Yeast)
ExpressionBacterialAvailable SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only