We narrowed to 6,087 results for: nls

1. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS42K_MCP-VP64_D-MS2

   Plasmid

   #177161

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   D-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS42K_PCP-VP64_A-PP7

   Plasmid

   #177156

   Purpose

   Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   PCP-VP64
   A-PP7
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS42K_MCP-VP64_C-MS2

   Plasmid

   #177160

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   C-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC0_4_VPR

   Plasmid

   #163716

   Purpose

   Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   VPR
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 4a)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TC512

   Plasmid

   #164888

   Purpose

   Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   zip-QFAD
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL1211

   Plasmid

   #129521

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFlag-NLS

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0008

   Plasmid

   #117535

   Purpose

   Level0 Golden Gate part: CDS, AsCas12a

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   AsCas12a (with stop codon)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDS2

   Plasmid

   #70135

   Purpose

   CEN/ARS yeast shuttle vector with DS2 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   AgTEF1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL4.23-CD69 CaRE3 (minus strand)

    Plasmid

    #91845

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0622)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-IL2RA CaRE4 (plus strand)

    Plasmid

    #91850

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0345)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-IL2RA CaRE4 (minus strand)

    Plasmid

    #91849

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0626)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4.23-IL2RA CaRE1 (plus strand)

    Plasmid

    #91837

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0614)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.23-CD69 CaRE3 (plus strand)

    Plasmid

    #91844

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0621)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23-IL2RA CaRE5 (plus strand)

    Plasmid

    #91841

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0618)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23-IL2RA CaRE3 (minus strand)

    Plasmid

    #91835

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0612)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE3 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

    Promoter

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.23-IL2RA CaRE4 SNP (plus strand)

    Plasmid

    #91851

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0346)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs61839660 C>T

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.23-IL2RA CaRE5 (minus strand)

    Plasmid

    #91836

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0613)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.23-IL2RA CaRE2 (plus strand)

    Plasmid

    #91846

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0623)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs6602425 T>G, T>C at chr10:6117603

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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