We narrowed to 5,690 results for: org

1. pBFC0687

   Plasmid

   #186692

   Purpose

   ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsB
   tnsC
   tniQ
   Cas12k
   Sh_2xBsaI_NT (Guide Stuffer) gRNA
   Tn7 transposon
   GmR+sfGFP ShDART Cargo
   

   Expression

   Bacterial

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

   Plasmid

   #91885

   Purpose

   Retroviral expression of mouse Ezh2 with a SET domain deletion

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   SET domain deleted

   Promoter

   MMLV gag

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_GFP10-14-SEPT7

   Plasmid

   #180341

   Purpose

   mammalian expression of human SEPT7 fused to GFP10

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN7 (SEPTIN7 Human)
   

   Tags

   GFP10

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

   Plasmid

   #91879

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

6. Tol2-pA-GI-TagRFP-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

   Plasmid

   #135812

   Purpose

   This plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

   Depositor

   Nadia Mercader Huber

   Article

   Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

   Insert

   TagRFP
   

   Use

   Zebrafish

   Promoter

   UAS

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBFC0694

   Plasmid

   #186693

   Purpose

   ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsB
   tnsC
   tniQ
   Cas12k
   Sh_lacZ_α_1 gRNA
   Tn7 transposon
   GmR+sfGFP ShDART Cargo
   

   Expression

   Bacterial

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. S10A mutant of H3S10ph biosensor

   Plasmid

   #120810

   Purpose

   FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
   

   Expression

   Mammalian

   Mutation

   Serine 10 is mutated to Alanine, and Threonine 3,…

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

   Plasmid

   #91880

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pPB-cT3G-cERP2-EMX2

    Plasmid

    #192898

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of EMX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    EMX2 (EMX2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_SEPT9_i1-14-GFP10

    Plasmid

    #180343

    Purpose

    mammalian expression of human SEPT9_i1 fused to GFP10

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    GFP10

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX GFP Mps1 DK

    Plasmid

    #63703

    Purpose

    Inducible expression of GFP-Mps1 DK fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of aa 47-156, kinetochore binding domain

    Promoter

    tight TRE promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-JUN

    Plasmid

    #192895

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of JUN

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    JUN (JUN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Expression

    Mammalian

    Mutation

    No stop codon

    Available Since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLIB_GST-nsp13 (SARS-CoV-2)

    Plasmid

    #169190

    Purpose

    Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    GST

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPTH OROV N

    Plasmid

    #229663

    Purpose

    Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag

    Depositor

    Stephen Graham

    Article

    Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

    Insert

    Nucleoprotein
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid

    #222288

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis, GFP, and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

    Plasmid

    #222289

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6His and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-SAM-Puro

    Plasmid

    #198713

    Purpose

    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Inserts

    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a and U6

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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