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pEF Doc2alpha D291N mutant GFP

Plasmid

#128821

Purpose

To visualize Doc2alpha D291N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D291N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta D303N mutant GFP

Plasmid

#128822

Purpose

To visualize Doc2beta D303N mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

D303N

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro NM23 H1 Pro

Plasmid

#11366

Depositor

Ronald Kahn

Article

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Pro96 mutation to Ser (reduces GAP activity).

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Vinculin-23

Plasmid

#55100

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vinculin (VCL Human)

Tags

mCherry and miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
USH1C_PDZ_1

Plasmid

#103936

Purpose

Protein expression and purification of P73 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

USH1C (USH1C Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-0-CFP

Plasmid

#41664

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-224N

Plasmid

#31634

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

1st 224 amino acids of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (AAA)

Plasmid

#24241

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pTRC-APX(W41F) dimeric

Plasmid

#58499

Purpose

Bacterial expression of APX W41F inducible by IPTG

Depositor

Alice Ting

Article

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F (dimeric)

Tags

His6

Expression

Bacterial

Mutation

W41F

Promoter

trc

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFAB3665

Plasmid

#47832

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 1 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3701

Plasmid

#47835

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 4 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3845

Plasmid

#47840

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 9 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3857

Plasmid

#47841

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 10 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1902

Plasmid

#47858

Purpose

BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

M13_central_T_linker_rrnD_T1 double terminator

Use

Synthetic Biology

Expression

Bacterial

Mutation

concatenate of M13 central and rrnD

Promoter

pLTetO13

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3997

Plasmid

#47828

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD21 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD21

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3912

Plasmid

#47815

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD8 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD8

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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