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Showing: 7321 - 7360 of 10228 results

  3. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  5. MRGPRD-DuET

    Plasmid
    #213340
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRD (MRGPRD Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213340-plasmid-map-sequence-id-430868

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  8. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Use
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  12. OPRD1-DuET

    Plasmid
    #213358
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRD1 (OPRD1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213358-plasmid-map-sequence-id-430880

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  21. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  22. RXFP1-DuET

    Plasmid
    #213373
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    RXFP1 (RXFP1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213373-plasmid-map-sequence-id-430890

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  24. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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Showing: 7321 - 7360 of 10228 results