We narrowed to 10,449 results for: T7
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TAL3165
Plasmid#41339DepositorInsertZebrafishCommunity-slc26a6-Right (LOC100005108 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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TAL3164
Plasmid#41338DepositorInsertZebrafishCommunity-slc26a6-Left (LOC100005108 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub-RLuc8N-NES
Plasmid#138213PurposeEncodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS1
Plasmid#145362PurposeCell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherryDepositorInsertZIKV NS1
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA2_ZIKV NS2B
Plasmid#145364PurposeCell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherryDepositorInsertZIKV NS2B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS3
Plasmid#145365PurposeCell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherryDepositorInsertZIKV NS3
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-17b_K490clm_E215C
Plasmid#169995PurposeKinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labelingDepositorInsertkinesin family member 5B, isoform CRA_a
UseTags6xHISExpressionBacterialMutationC7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-CKAR2
Plasmid#236097PurposeCytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertExRai-CKAR2-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4/5FTrp)RS
Plasmid#207620PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-alpha-synuclein-BiFC
Plasmid#87855PurposepETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisationDepositorInsertsalpha-synuclein-VC155
alpha-synuclein-VN154
UseTagsC-terminal fragment of mVenus BiFC protein and N-…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
UseTagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-OSF-CypA
Plasmid#79039PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertOneSTrEP-FLAG- Cyclophilin A (PPIA Human)
UseTagsOneSTrEP-FLAGExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseCRISPRTags6XHis, HA, and SV40 NLSExpressionBacterialMutationPromoterT7Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUBA1
Plasmid#63571PurposeExpression of human UBA1 tagged with HisDepositorInsertUBA1 (UBA1 Human)
UseTagsExpressionBacterialMutationIsoform 2 (1018 amino acids)PromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETduet+sfGFP-TetR-p300HAT
Plasmid#157795PurposeExpression of sfGFP-TetR-p300HAT fusion proteinDepositorInsertsfGFP-TetR-p300 (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsHisExpressionBacterialMutationWhole proteinPromoterT7Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NES
Plasmid#175015PurposeCytosol-targeted TORCAR.DepositorInsertTORCAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin (1-76; T66V, L67N)
Plasmid#110755PurposeBacterial expression of human Ubiquitin (1-76; T66V, L67N)DepositorInsertUBB (UBB Human)
UseTagsExpressionBacterialMutationT66V, L67NPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DNAseI
Plasmid#182408PurposeBacterial expression of DNaseIDepositorInsertDNase I (DNASE1 Bovine)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
