We narrowed to 18,759 results for: nls
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Plasmid#198890PurposeExpress AID(1-188) BE3-based base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertActication induced deaminase (AICDA Human)
TagsNLSExpressionMammalianMutationDeletion of amino acids 189-198PromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HeFSpCas9-NLS-6xHis
Plasmid#207392PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-evoSpCas9-NLS-6xHis
Plasmid#207390PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-evoSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationM495V, Y515N, K526E, R661Q, amino acids 1005-1013…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-NLS-3XFLAG-V5
Plasmid#196089PurposeExpresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mcGAS-LL/RK-NLS/D307A
Plasmid#185459PurposeInducible nuclear cGAS with D307A mutationDepositorPublicationInsertcGAS
UseLentiviralTagsFLAG and SV40 NLSExpressionMammalianMutationL157R, L159K, D307AAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdPCP-GFP-VPR
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-QUE37C/pLBS CAG
Plasmid#129322PurposeATP measurement, nucleus, lentivirusDepositorPublicationInsertQUEEN-37C
UseLentiviralAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD355 VPR-FRB (NLS)
Plasmid#138847PurposeConstitutive expression of VPR-FRB under the CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVPR-FRB
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS
Plasmid#129530PurposeSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3NmeDepositorInsertscodon-optimized AcrIIC3Nme
sgRNA_Rosa26
UseAAVTagsFLAG/NLSPromoterU6 and cytomegalovirus-enhancer chicken β-actin p…Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
Plasmid#114078PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
TagsSV40 NLSExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65
Plasmid#104566PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1(D832A)-p65
Tags3x HA and NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3xSNAP-NLS
Plasmid#236388PurposeOverexpression of PCP-3xSNAP in human cellsDepositorInsertPCP-3xSNAP
UseLentiviralAvailable sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-NLS-Beta-catenin-S33Y
Plasmid#138537PurposeFor making Beta-catenin-S33Y RNADepositorInsertBeta-catenin
UseIn vitro transcriptionTags2XFLAG and 2xNLSMutationchanged serine 33 to tyrosinePromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3
Plasmid#122092PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus localizedDepositorInsertrsCaMPARI
UseAAVTagsNLS-His and mRuby3Promoterhsyn (synapsin-1)Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
Plasmid#127365PurposeBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry
Plasmid#203652PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.DepositorPublicationInsertPfV
UseLentiviralTagsSapphire-IRES-H2B-mCherryAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-MKII::nlsLexADBDo
Plasmid#64718Purposegenerating transgenic TRIC fliesDepositorInsertMKII::nlsLexADBDo
ExpressionInsectAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only