We narrowed to 6,501 results for: nls
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Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1518
Plasmid#205981PurposeFLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHS1514
Plasmid#205977PurposeFLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas8/Cse1 from MWEE01000013, Cas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1515
Plasmid#205978PurposeFLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas11/Cse2 from MWEE01000013, Cas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas7 from MWEE01000013, Cas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_MCS_version2
Plasmid#197950PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRExpressionPlantPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
TagsHuman EMD transmembrane domain and NLSExpressionMammalianPromoterCAGAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GWF303
Plasmid#133611PurposeCD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFPDepositorInsertCD95-1-2xPP7, PCP-DNCR2, BFP
ExpressionMammalianAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF314
Plasmid#133610PurposeCXCR4-com, NLS-com-GNCR1-P2a-BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR4-6-com, com-GNCR1, BFP
ExpressionMammalianAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUra4-Pmad3-MCP-2xNLS-NES-tdSG
Plasmid#255014Purposeexpression of MCP-2xNLS-NES-tandemStayGold from weak P.mad3 promoterDepositorInsertP.mad3(717-1)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1
ExpressionYeastPromoterSpom\P.mad3Available sinceJune 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUra4-Pmad3-MCP-2xNLS-2xNES-tdSG
Plasmid#255015Purposeexpression of MCP-2xNLS-2xNES-tandemStayGold from weak P.mad3 promoterDepositorInsertP.mad3(717-1)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1
ExpressionYeastPromoterSpom\P.mad3Available sinceJune 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUra4-Pcdc2short-MCP-2xNLS-NES-tdSG
Plasmid#255016Purposeexpression of MCP-2xNLS-NES-tandemStayGold from medium-strength P.cdc2 promoterDepositorInsertP.cdc2(355-1,AfeImut)-MCP-2xNLS-NES-td8ox2StayGold-Scer\T.ADH1
ExpressionYeastMutation1 nucleotide removed in cdc2 promoter to abolish …PromoterSpom\P.cdc2Available sinceJune 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUra4-Pcdc2short-MCP-2xNLS-2xNES-tdSG
Plasmid#255017Purposeexpression of MCP-2xNLS-2xNES-tandemStayGold from medium-strength P.cdc2 promoterDepositorInsertP.cdc2(355-1,AfeImut)-MCP-2xNLS-2xNES-td8ox2StayGold-Scer\T.ADH1
ExpressionYeastMutation1 nucleotide removed in cdc2 promoter to abolish …PromoterSpom\P.cdc2Available sinceJune 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476)
Plasmid#248216PurposebpNLS-BxbI IVT vector for mRNA productionDepositorInsert5'UTR-bpNLS-BxbI-3'UTR-polyA
UseCRISPR; In vitro transcriptionTagsBPNLSExpressionMammalianMutationNonePromoterT7Available sinceMay 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
UAST-FRT-GFP-FRT-uMCas12a+nls1x
Plasmid#230908PurposeDouble inducible expression of Cas12a+ with Gal4 and Flp.DepositorInsertCas12a+
UseCRISPRExpressionInsectAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-(G2SG2)2-Cre (JDW 1149)
Plasmid#224514PurposeA Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to CreDepositorInsert3xNLS-(G2SG2)2-Cre
UseCre/LoxAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-eBE
Plasmid#107688PurposeExpresses dLbCpf1-eBE in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1-eBE
ExpressionMammalianMutationdLbCpf1(D832A/E925A/D1148A)PromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRQR variant
Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
frq[ΔNLS, mNG_V5]_nat-1
Plasmid#251399PurposeEncodes an NLS1-deleted frq allele with C-terminal mNeonGreen and V5 tags, enabling fluorescent visualization and detection following replacement of the endogenous frq locus.DepositorInsertfrq
UseNeurospora expressionTagsV5 and mNeonGreenMutationdeleted amino acids 95-100Available sinceMay 27, 2026AvailabilityAcademic Institutions and Nonprofits only