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pRetroX GFP Mps1 wt

Plasmid

#63702

Purpose

Inducible expression of GFP-Mps1 fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCherry-Rab8A

Plasmid

#194333

Purpose

For use in light-induced Rab8a protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab8a (RAB8A Canis Lupus)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

Plasmid

#145840

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-Venus

Plasmid

#153950

Purpose

piggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion protein

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MYC

Plasmid

#192899

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of MYC

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

MYC (MYC Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZBTB16

Plasmid

#192903

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ZBTB16 (ZBTB16 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

Plasmid

#139444

Purpose

Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

sequence codon-optimized

Promoter

SFFV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Ad:2CMV_A2R2-N25K3

Plasmid

#122242

Purpose

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2E

Plasmid

#224441

Purpose

Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQGYQ insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3D

Plasmid

#224445

Purpose

Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYREL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3E

Plasmid

#224446

Purpose

Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4B

Plasmid

#224449

Purpose

Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4D

Plasmid

#224451

Purpose

Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHGVL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4C

Plasmid

#224450

Purpose

Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYTSM insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3C

Plasmid

#224444

Purpose

Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSSV insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3B

Plasmid

#224443

Purpose

Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYSGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 WT

Plasmid

#158541

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHOT-P2Y2R

Plasmid

#159108

Purpose

entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

Depositor

Fabio Mammano

Article

Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

Insert

P2Y2R

Use

Lentiviral

Promoter

UBC

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBFC0625

Plasmid

#186691

Purpose

VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Mutation

G12V

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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