We narrowed to 9,400 results for: Coli

1. pCMK188

   Plasmid

   #226659

   Purpose

   pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site

   Depositor

   David Nielsen

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T-3-6AOptParkin

   Plasmid

   #165107

   Purpose

   bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene

   Depositor

   Arthur Haas

   Insert

   Parkin isoform 1 (PRKN Human)
   

   Use

   Tags

   GST-6-alanine

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. A3RXB7

   Plasmid

   #177694

   Purpose

   expresses a N-acetyl hexosamine oxidase in E. coli

   Depositor

   Martin Engqvist

   Article

   Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

   Insert

   N-acetylhexosamine oxidase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTGD

   Plasmid

   #88894

   Purpose

   Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

   Depositor

   Keith Tyo

   Article

   Love et al Methods Mol Biol. 2019;1927:37-45. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIB190

   Plasmid

   #90197

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-a(+)-6xHis-LSSmCherry1(-13)

   Plasmid

   #219415

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   LSSmCherry1(-13)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-c5X-GLP-1

   Plasmid

   #66997

   Purpose

   Expresses malE-GLP-1 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-GLP-1
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-6His-SUMO-Nsp14

   Plasmid

   #154956

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pG1AK

   Plasmid

   #71736

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIB168

    Plasmid

    #90191

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRL488

    Plasmid

    #70263

    Purpose

    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

    Depositor

    Peter Wolk

    Article

    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

    Type

    Empty backbone

    Use

    Luciferase; Cyanobacteria expression

    Tags

    luxAB

    Expression

    Mutation

    Promoter

    none

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIB164

    Plasmid

    #90187

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcrRNA.con

    Plasmid

    #61285

    Purpose

    Constitutive repeat-only E. coli CRISPR array

    Depositor

    Chase Beisel

    Article

    Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIB167

    Plasmid

    #90190

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIB169

    Plasmid

    #90192

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL-c5X-SELP0K

    Plasmid

    #67007

    Purpose

    Expresses malE-SELP0K in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-SELP0K
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIB163

    Plasmid

    #90186

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMAL-c5X-Darpin E_01

    Plasmid

    #66996

    Purpose

    Expresses malE-Darpin E_01 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-Darpin E_01
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKG113

    Plasmid

    #63073

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-T (CENPT Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized. G217R mutation compared …

    Promoter

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-c5X-poly-RGD

    Plasmid

    #67002

    Purpose

    Expresses malE-poly-RGD in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-poly-RGD
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX6P1 pS43 METTL3

    Plasmid

    #200732

    Purpose

    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3

    Depositor

    Claudio Alarcón

    Insert

    pS43 METTL3 (METTL3 Human)
    

    Use

    Tags

    GST from backbone plasmid

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli, UAG co…

    Promoter

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pGL4.26-SS-358

    Plasmid

    #68790

    Purpose

    ZF9 Op x6 -- NTR

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9 Op x6
    Nitroreductase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-c5X-BRT17

    Plasmid

    #67000

    Purpose

    Expresses malE-BRT17 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-BRT17
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUCX

    Plasmid

    #60681

    Purpose

    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression

    Depositor

    David Ackerley

    Article

    Prosser et al Biotechnol Lett. 2014 Sep 26.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSETA_ionRE

    Plasmid

    #172926

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUCXKT

    Plasmid

    #60682

    Purpose

    positive selection cloning and expression vector

    Depositor

    David Ackerley

    Article

    Prosser et al Biotechnol Lett. 2014 Sep 26.

    Type

    Empty backbone

    Use

    Positive selection e. coli expression vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28-Strep-TEV-Nsp14

    Plasmid

    #154955

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. enz.079-GCN5

    Plasmid

    #154046

    Purpose

    Expresses GCN5 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone acetyltransferase GCN5 (GCN5 Budding Yeast)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge