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We narrowed to 14,145 results for: TIM

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Showing: 9201 - 9250 of 14145 results

  12. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid
    #239461
    Purpose
    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239461-plasmid-map-sequence-id-476832

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  13. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid
    #218198
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM
    Depositor
    Florence Pojer
    Insert
    EndoCOM
    Tags
    3C-CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 19-315
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218198-plasmid-map-sequence-id-431794

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  14. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211339
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211339-plasmid-map-sequence-id-415656

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  15. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  16. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  17. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid
    #211343
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211343-plasmid-map-sequence-id-415647

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  18. GBH-2aG4-gcryBFP.1

    Plasmid
    #188013
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188013-plasmid-map-sequence-id-381108

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  19. GBH-2aG4-gcryBFP.2

    Plasmid
    #188014
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188014-plasmid-map-sequence-id-381045

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  20. GBH-2aG4-gcryGFP.1

    Plasmid
    #188016
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188016-plasmid-map-sequence-id-381139

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  21. GBH-2aG4-gcryGFP.2

    Plasmid
    #188017
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188017-plasmid-map-sequence-id-381140

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  22. GBH-2aG4-gcryGFP.3

    Plasmid
    #188018
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available Since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188018-plasmid-map-sequence-id-381141

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  23. pT7CFE1_EVD68_VP12A_C107R

    Plasmid
    #212982
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A C107R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed cysteine 107 from 2A to Arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212982-plasmid-map-sequence-id-422577

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  24. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

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  25. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid
    #212980
    Purpose
    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABCD C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed cysteine 147 from 3C to arginine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212980-plasmid-map-sequence-id-420353

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  26. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

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  27. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid
    #203710
    Purpose
    Overexpression of FLAG-S1PR1-S5A mutant.
    Depositor
    Timothy Hla
    Insert
    Flag-hS1PR1-S5A (S1PR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S5A
    Promoter
    CMV
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203710-plasmid-map-sequence-id-403867

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  28. pET3-ARL1-His6 (CO)

    Plasmid
    #201866
    Purpose
    Used for bacterial expression of human protein ARL1
    Depositor
    Richard Kahn
    Insert
    ARL1 (ARL1 Human)
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201866-plasmid-map-sequence-id-403817

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  29. pET3-[Δ1-32]ARL10-His6(CO)

    Plasmid
    #201876
    Purpose
    Used for bacterial expression of human protein ARL10
    Depositor
    Richard Kahn
    Insert
    ARL10 (ARL10 Human)
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Deletion of residues 1-32 from N-terminus and cod…
    Available Since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201876-plasmid-map-sequence-id-403811

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  30. pET3-ARF4-His6(CO)

    Plasmid
    #201885
    Purpose
    Used for bacterial expression of human protein ARF4
    Depositor
    Richard Kahn
    Insert
    ARF4 (ARF4 Human)
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201885-plasmid-map-sequence-id-403153

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  31. pET3-ARF3-His6(CO)

    Plasmid
    #201884
    Purpose
    Used for bacterial expression of human protein ARF3
    Depositor
    Richard Kahn
    Insert
    ARF3 (ARF3 Human)
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201884-plasmid-map-sequence-id-402538

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  32. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

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  33. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

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  34. pHH0103 CEP164 WW domain

    Plasmid
    #104276
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104276-plasmid-map-sequence-id-203766

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  35. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104300-plasmid-map-sequence-id-203483

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  36. pHH0103 HECW2 WW domain #1

    Plasmid
    #104244
    Purpose
    Bacterial expression of WW domain #1 from HECW2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HECW2 WW domain #1 (HECW2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104244-plasmid-map-sequence-id-204378

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  45. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  48. pCMV-T7-ABEmax-SpRY

    Plasmid
    #195279
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195279-plasmid-map-sequence-id-394869

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Showing: 9201 - 9250 of 14145 results