We narrowed to 7,201 results for: mme

1. pKD284

   Plasmid

   #125073

   Purpose

   Expression of SO_4488 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4488 (SO_4488 )
   lacI (lacI S286L- Please see depositor comments below)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD278

   Plasmid

   #125070

   Purpose

   Expression of SO_2192 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_2192 (SO_2192 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Mutation in LacI S286L- Please see depositor comm…

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOU2

   Plasmid

   #41754

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc E. coli, Fly)
   

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S C451S

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-ecDHFR-dCys-T46C

   Plasmid

   #110543

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   T46 mutated to C46, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-ecDHFR-dCys-L54C

   Plasmid

   #110542

   Purpose

   Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   L54 mutated to C54, C85 mutated to A85, C152 muta…

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

   Plasmid

   #110159

   Purpose

   Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E135A/S139A

   Promoter

   CMV

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT3-RhoGC D380E

   Plasmid

   #100035

   Purpose

   This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

   Depositor

   Daniel Oprian

   Article

   Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

   Insert

   RhoGC D380E
   

   Tags

   1D4 (TETSQVAPA)

   Expression

   Mammalian

   Mutation

   D380E

   Promoter

   Adenovirus major late promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMT3-RhoGC D380N

   Plasmid

   #100034

   Purpose

   This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

   Depositor

   Daniel Oprian

   Article

   Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

   Insert

   RhoGC D380N
   

   Tags

   1D4 (TETSQVAPA)

   Expression

   Mammalian

   Mutation

   D380N

   Promoter

   Adenovirus major late promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PRR4-bio-His

    Plasmid

    #52075

    Purpose

    Expresses full-length Proline-rich protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PRR4 (PRR4 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TM9-bio-His

    Plasmid

    #51831

    Purpose

    Expresses full-length Transmembrane 9 superfamily member 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TM9 (TM9SF4 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. G6B-bio-His

    Plasmid

    #51644

    Purpose

    Expresses full-length Protein G6b precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    G6B (MPIG6B Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-hRIP1-FL I541P

    Plasmid

    #61381

    Purpose

    Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I541P mutation (RIPK1 Human)
    

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I541 to P

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pTYB-pLAC-F112S

    Plasmid

    #48727

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Phenylalanine 12 to Serine

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pTYB1-pLAC-I98S

    Plasmid

    #48723

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 98 to Serine

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pTYB1-pLAC-I73S

    Plasmid

    #48722

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 73 to Serine

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pTYB1-pLAC-V69S

    Plasmid

    #48721

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Valine 69 to Serine

    Promoter

    T7

    Available Since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCR4-*mRFP

    Plasmid

    #47118

    Purpose

    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    *mRFP
    

    Expression

    Bacterial

    Mutation

    No start codon

    Promoter

    Lac

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDM10

    Plasmid

    #41759

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc E. coli, Fly)
    

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S A378C C451S

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge