We narrowed to 10,761 results for: T7

Showing: 7401 - 7440 of 10761 results

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pACYCDuet_L54IDHFR_R166QTS

Plasmid

#91234

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_L54IDHFR_WTTS

Plasmid

#91235

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

L54I

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FDHFR_R166QTS

Plasmid

#91236

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45 (delta 154–164)

Plasmid

#126879

Purpose

Expresses crystallisation construct of human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

Human Cdc45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Mutation

deletion amino acid 154-164

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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B. adolescentis mRNA toehold switch sensor

Plasmid

#110710

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-YJR129c

Plasmid

#85118

Purpose

bacterial expression of YJR129c

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFM3 (EFM3 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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F. prausnitzii mRNA toehold switch sensor

Plasmid

#110716

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

F. prausnitzii species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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C. difficile mRNA toehold switch sensor

Plasmid

#110714

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
R. hominis mRNA species specific sensor

Plasmid

#110713

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

R. hominis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E. rectale mRNA toehold switch sensor

Plasmid

#110712

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. rectale species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. breve mRNA toehold switch sensor

Plasmid

#110711

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. breve species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. longum mRNA toehold switch sensor

Plasmid

#110709

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. longum species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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E. coli mRNA toehold switch sensor

Plasmid

#110708

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. coli species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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F. prausnitzii 16S toehold switch sensor

Plasmid

#110705

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

F. prausnitzii 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
R. hominis 16S toehold switch sensor

Plasmid

#110703

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

R. hominis 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E. rectale 16S toehold switch sensor

Plasmid

#110702

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. rectale 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. breve 16S toehold switch sensor

Plasmid

#110701

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. breve 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. adolescentis 16S toehold switch sensor

Plasmid

#110700

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. longum 16S toehold switch sensor

Plasmid

#110699

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. longum 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. thetaiotaomicron 16S toehold switch sensor

Plasmid

#110697

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. thetaiotaomicron 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7T3-PacI_MafB_ISH

Plasmid

#87670

Purpose

In situ probe for mouse Mafb

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mafb (Mafb Mouse)

Expression

Bacterial

Promoter

T7/T3

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; F4A)

Plasmid

#110757

Purpose

Bacterial expression of Ubiquitin (1-76; F4A)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

F4A

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys

Plasmid

#110541

Purpose

Bacterial protein of E.coli DHFR C85A/C152S mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

C85 mutated to A85, C152 mutated to S152

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP

Plasmid

#110828

Purpose

Bacterial expression of E.coli DHFR N23PP mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b+R3(R112C/T180C)C

Plasmid

#108194

Purpose

Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C)-P450cam fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b+3(R112C/T180C)C

Plasmid

#108193

Purpose

Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA3(R112C/T180C)-P450cam fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+2(L171C)X

Plasmid

#108192

Purpose

Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA2(L171C)-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

L171C (PCNA2); C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+R2(L171C)X

Plasmid

#108191

Purpose

Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C)-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

L171C (PCNA2); C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+C3(R112C/T180C)X

Plasmid

#108196

Purpose

Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

P450cam-PCNA3(R112C/T180C)-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3); C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+1(G108C)X

Plasmid

#108189

Purpose

Expresses PCNA1(G108C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1(G108C)-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

G108C (PCNA1); C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - MPX

Plasmid

#89619

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Mitocondrial Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - EtfB

Plasmid

#89617

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

electron-transfer-flavoprotein, alpha polypeptide

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - TDR

Plasmid

#89627

Purpose

Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

thiol dependent reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - NFDD

Plasmid

#89624

Purpose

Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD/FAD dependent dehydrogenase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET151-Vps4(101-437) L151D

Plasmid

#87736

Purpose

Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Leu 151 to Asp

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 10,761 results for: T7