Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,125 results for: golden gate

Showing: 3701 - 3720 of 5125 results

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pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_AsLOV2-S103ΔELS

Plasmid

#246049

Purpose

Expression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_AsLOV2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_cpGR2(GGS5)-S103ΔELS

Plasmid

#246050

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

Plasmid

#246051

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIC3_cpGR2(GGS5)-F59

Plasmid

#246053

Purpose

Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIC3_cpGR2-F59

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_F113L

Plasmid

#248016

Purpose

Expression of synthetic Kemp eliminase (Des27.7 F113L)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_F113L

Tags

12xHis-bdSumo

Expression

Bacterial

Mutation

F113L

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27

Plasmid

#247996

Purpose

Expression of synthetic Kemp eliminase (Des27)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA1751

Plasmid

#246066

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori

Depositor

Jennifer Doudna

Article

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Panoptes locus

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3701 - 3720 of 5125 results

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