We narrowed to 3,807 results for: golden gate
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Plasmid#138100PurposeGolden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGGG-AH-VRT-A2
Plasmid#163703PurposeWheat transformation vector pGoldenGreenGate (pGGG) with OsActinP:: hygromycin (hpt) selection and the full native Triticum polonicum VRT-A2 gene (native prom::genomic seq::3'UTR)DepositorInsertsOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
ExpressionPlantPromoterRice - Os Actin promoter and Triticum polonicum V…Available SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUN378 - pTRV2-CrGLK
Plasmid#203888PurposeViral silencing plasmid targeting the CrGLK gene from C. roseus.DepositorInsertCrGLK fragment
UseRNAiExpressionPlantAvailable SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUN381 - pTRV2-CHLH
Plasmid#203886PurposeViral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.DepositorInsertCHLH fragment
UseRNAiExpressionPlantAvailable SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK3071
Plasmid#219755PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertmcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
ExpressionPlantAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK497
Plasmid#219754PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.DepositorInsertnnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
ExpressionPlantAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB.PRC2
Plasmid#234579PurposeExpression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4DepositorAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNK115
Plasmid#219745PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK116
Plasmid#219742PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamianaDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
Plasmid#218910PurposePiggyBac transpositionDepositorInsertV5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
ExpressionMammalianMutation2363G>C and 4155C>T and 4729T>G and 4731…Available SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK189
Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK845
Plasmid#219746PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiensDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227898PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertLAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227899PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227890PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertTOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227907PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227923PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227908PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227909PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only