We narrowed to 117,517 results for: mpi

1. T7RNAP Cyslight A188C

   Plasmid

   #205083

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Insert

   T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S N88C

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSOX2.1.0-gDNA

   Plasmid

   #176382

   Purpose

   Expresses spCas9 and gRNA sequence targeting SOX2

   Depositor

   Kevin White, Barbara Stranger

   Insert

   SOX2 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPOII p48 (DM#261)

   Plasmid

   #15026

   Depositor

   Douglas Melton

   Insert

   p48 (Ptf1a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pPs-neo HA-RalA

   Plasmid

   #20181

   Depositor

   William Hahn

   Insert

   RalA (RALA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G23V (constitutively active), E147D

   Promoter

   Available Since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-EcoRIM

   Plasmid

   #165515

   Purpose

   Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   M.EcoRI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. lenti.Cas9.BFP.sgRNA.parental

   Plasmid

   #196715

   Purpose

   Sortable WT-SpCas9 expression and guide cassette (1-vector system).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO_mCherry_AAVS1

   Plasmid

   #141212

   Purpose

   pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Insert

   gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB-Bleo STAT3

   Plasmid

   #139184

   Purpose

   Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.

   Depositor

   Neal Devaraj

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   STAT3

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP019

   Plasmid

   #168195

   Purpose

   expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.

   Depositor

   James Fleckenstein

   Insert

   eatA[H134R]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H134R mutation

   Promoter

   araBAD

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1-His-NEDD8

    Plasmid

    #159322

    Purpose

    Mammalian cell expression of mouse His-NEDD8

    Depositor

    Jianjun Zhao

    Insert

    His-NEDD8 (Nedd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST40-DJ1-C106A

    Plasmid

    #29409

    Depositor

    Mark Cookson

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBSVSG221KOblast

    Plasmid

    #59733

    Purpose

    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.

    Depositor

    Gloria Rudenko

    Insert

    VSG221 knockout construct
    

    Use

    For homologous recombination into vsg221 expressi…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS1 - pRig-3::GCaMP6f::unc-54

    Plasmid

    #194970

    Purpose

    Fluorescent reporter of cytosolic calcium in C.elegans.

    Depositor

    Frederic Hoerndli

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rig-3

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM263-EcPPK

    Plasmid

    #38333

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. ADH1pro_GCN4_SIR4

    Plasmid

    #138620

    Purpose

    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

    Depositor

    Rob Mitra

    Insert

    GCN4 (GCN4 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid

    #92296

    Purpose

    Expression of humanized FbCpf1

    Depositor

    Feng Zhang

    Insert

    huFbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-mTie1

    Plasmid

    #119234

    Purpose

    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    Tie1 (Tie1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    P hUbC

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MT1A

    Plasmid

    #105700

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1a

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1a (MT1A Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SEMA3E (mouse) pBLUE

    Plasmid

    #29453

    Depositor

    Jonathan Raper

    Insert

    Sema3E (mouse) (Sema3e Mouse)
    

    Use

    Pblue for ish

    Tags

    Expression

    Mutation

    1400bp fragment for ISH

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. L3691

    Plasmid

    #1587

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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