Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,474 results for: BLI;

Clear keywords
Showing: 7441 - 7480 of 9474 results

  1. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid
    #215544
    Purpose
    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus
    Depositor
    Kai Wang
    Insert
    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    Use
    CRISPR
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    215544-plasmid-map-sequence-id-424979

    Enlarge

  3. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

    Enlarge

  4. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

    Enlarge

  6. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

    Plasmid
    #192113
    Purpose
    Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-NTD-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192113-plasmid-map-sequence-id-374987

    Enlarge

  7. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid
    #164055
    Purpose
    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164055-plasmid-map-sequence-id-327673

    Enlarge

  8. mouse KANK3 Ankyrin Repeats pET151

    Plasmid
    #164056
    Purpose
    Expresses mouse KANK3 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164056-plasmid-map-sequence-id-327670

    Enlarge

  9. mouse KANK4 Ankyrin Repeats pET151

    Plasmid
    #164057
    Purpose
    Expresses mouse KANK4 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164057-plasmid-map-sequence-id-327675

    Enlarge

  11. pCDZ2FE_Champ1_1-812_2PA

    Plasmid
    #105676
    Purpose
    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P336A, P341A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105676-plasmid-map-sequence-id-203181

    Enlarge

  12. pT5T_Rev7_1-211_SA112_KVK_YAK

    Plasmid
    #105630
    Purpose
    Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3
    Depositor
    Thanos Halazonetis
    Insert
    Rev7 (Mad2l2 Mouse)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105630-plasmid-map-sequence-id-203820

    Enlarge

  13. MS2_SC2_S_B.1.640

    Plasmid
    #180228
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    B.1.640 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180228-plasmid-map-sequence-id-352513

    Enlarge

  14. CD44U-c010

    Plasmid
    #175453
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175453-plasmid-map-sequence-id-353218

    Enlarge

  15. MS2_SC2_S_C.1.2

    Plasmid
    #177367
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    C1.2 (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177367-plasmid-map-sequence-id-347828

    Enlarge

  16. MS2_SC2_S_Mu

    Plasmid
    #177366
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Mu (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 20, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177366-plasmid-map-sequence-id-347825

    Enlarge

  17. MS2_SC2_S_Kappa

    Plasmid
    #175953
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Kappa (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175953-plasmid-map-sequence-id-343945

    Enlarge

  18. MS2_SC2_S_Eta

    Plasmid
    #175954
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Eta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175954-plasmid-map-sequence-id-343942

    Enlarge

  19. MS2_SC2_S_Gamma

    Plasmid
    #175047
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Gamma (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175047-plasmid-map-sequence-id-343946

    Enlarge

  20. MS2_SC2_S_Theta

    Plasmid
    #175951
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Theta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175951-plasmid-map-sequence-id-343941

    Enlarge

  21. MS2_SC2_S_Zeta

    Plasmid
    #175952
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Zeta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175952-plasmid-map-sequence-id-343947

    Enlarge

  22. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

    Enlarge

  23. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

    Enlarge

  25. pB6-Id1-T2A-tdTomato-FNF-TK

    Plasmid
    #139281
    Purpose
    B6J Id1-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Available Since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139281-plasmid-map-sequence-id-317908

    Enlarge

  26. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid
    #137044
    Purpose
    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    T2A-td-sfGFP
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    137044-plasmid-map-sequence-id-274424

    Enlarge

  30. Ad-mMKP3 S159A S197A-Flag

    Plasmid
    #131579
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    Flag
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available Since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131579-plasmid-map-sequence-id-256812

    Enlarge

  36. Myc-gamma1 (in pCAGGS)

    Plasmid
    #118039
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118039-plasmid-map-sequence-id-228636

    Enlarge

  37. pBABE.zeo/hKGA.S286A

    Plasmid
    #110357
    Purpose
    Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    KGA glutaminase (GLS Human)
    Use
    Retroviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S286A
    Promoter
    gag
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110357-plasmid-map-sequence-id-213568

    Enlarge

  38. His6-Halo-TEV-Akt2

    Plasmid
    #87606
    Purpose
    expresses His-halo tagged at the N-terminus of human Akt2 protein
    Depositor
    Yimon Aye
    Insert
    AKT 2 (AKT2 Human)
    Use
    Zebrafish (danio rerio) expression
    Tags
    His-Halo-Tev
    Expression
    Mammalian
    Available Since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87606-plasmid-map-sequence-id-197920

    Enlarge

  39. Hs.KRAS4b M72C

    Plasmid
    #83160
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M72C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    M72C
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    83160-plasmid-map-sequence-id-158123

    Enlarge

  40. Hs.KRAS4b R73K

    Plasmid
    #83163
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73K mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    R73K
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83163-plasmid-map-sequence-id-158126

    Enlarge

Showing: 7441 - 7480 of 9474 results