We narrowed to 10,522 results for: SEC
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Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3472
Plasmid#215245PurposeCDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathwayDepositorInsertH3H
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hy_pRnrS eGFP SV40
Plasmid#195062PurposeExpresses eGFP mRNA from the Drosophila RnrS promoterDepositorInserteGFP
ExpressionInsectPromoterRnrSAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hy_pAna eGFP SV40
Plasmid#195063PurposeExpresses eGFP mRNA from the Drosophila Ana promoterDepositorInserteGFP
ExpressionInsectPromoterAnaAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
TagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
H3-pAc5.1 OST4 mCherry Oskar 139-606
Plasmid#206494PurposeOskar 139-606 expression for Schneider cell imagingDepositorInsertOskar 139-606
TagsOST4 mcherryExpressionInsectAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-OH
Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
Tagsollas and his-6 added to C terminus of protein co…ExpressionInsectPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
F19-pAc5.1 EGFP Vasa 463-661
Plasmid#206486PurposeVasa 463-661 expression for Schneider cell imagingDepositorInsertVasa 463-661
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
F18-pAc5.1 EGFP Vasa 200-462
Plasmid#206485PurposeVasa 200-642 expression for Schneider cell imagingDepositorInsertVasa 200-462
TagsEGFPExpressionInsectAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE-3xStop
Plasmid#202151PurposeLevel 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.DepositorInsert3x Stop codon
UseSynthetic BiologyMutationNoneAvailable SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
OA-1067L
Plasmid#200253PurposepBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomatoDepositorInsert6 gRNAs targeting Doublesex, Intersex, bTublin
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1093B
Plasmid#194003PurposepBac-U6-crCHIKV-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting CHIKV
ExpressionInsectAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1085F
Plasmid#191375PurposepBac-U6-crYellow-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting yellow
ExpressionInsectAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-GFP::NMN-Deamidase
Plasmid#187868PurposeGal4/UAS expression of GFP::NMN-DeamidaseDepositorInsertNMN-Deamidase
TagsGFPExpressionInsectAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-CM
Plasmid#188585PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler Catalytic mutant
TagsFLAG-MycExpressionInsectMutationdeleted first ATG; D435A [GAT=>GCC]; E435A [GA…Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[N1-383]
Plasmid#188586PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler N-terminal domain
TagsFLAG-MycExpressionInsectMutationdeleted first ATGAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]
Plasmid#188587PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain
TagsFLAG-MycExpressionInsectAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1127B
Plasmid#190997PurposeExpresses arrays of gRNA targeting rel1 promoter under U6b/c promoterDepositorInsertU6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4
ExpressionInsectAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_1
Plasmid#190703PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 1
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_3
Plasmid#190705PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 3
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB202
Plasmid#169113PurposeTESEC expression vector for mEGFPDepositorInsertmEGFP
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRExpressionInsectAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S15-T79C
Plasmid#133048PurposeExpresses tagless S15-T79C mutant ribosomal protein in E.coliDepositorAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44I-c014
Plasmid#175451PurposeProtein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0764
Plasmid#177030PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertsecologanin synthase; CYP72C
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
ppBAalbNixE1E2E3E4 PUb-DsRed
Plasmid#173665Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
DsRed
ExpressionInsectMutationNix isoforms with 4 exonsAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD 1-147
Plasmid#165819Purpose1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertGal4BD 1-147
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL4AD 768-881
Plasmid#165820Purpose768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertGAL4AD 768-881
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Promoterless TP10
Plasmid#165787PurposeDrosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectorsDepositorInsertPromoterless T10
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Gal80
Plasmid#169030PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7746_v3
Plasmid#126856Purpose7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffoldsDepositorInsertII_CS3_7746_v3
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
ExpressionInsectAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6d-T
Plasmid#117212PurposeExpresses gRNA in Aedes aegyptiDepositorInsertu6d promoter-gRNA
UseSynthetic BiologyExpressionInsectPromoterU6Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only