We narrowed to 19,005 results for: Cre
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pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180267PurposeLentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNADepositorInsertEGFR sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-UFM1-dCS
Plasmid#139869PurposeMammalian expression of HA-tagged human UFM1-cCSDepositorInsertUFM1 (UFM1 Human)
UseTagsHAExpressionMammalianMutationd84-85PromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT9658-sgRNA-mCherry
Plasmid#162987PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD4
Plasmid#20186DepositorInsertNotch 4 Intracellular Domain (Notch4 Mouse)
UseTags3X FlagExpressionMammalianMutationContains murine Notch 4 Intracellular Domain (Val…PromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-2PH-PLCd-mKate2
Plasmid#109500PurposeMuscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.DepositorInsertactc1b-2PH-PLCd-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBK-VRER-BE3
Plasmid#85173PurposeExpresses VRER-BE3 in mammalian cellsDepositorInsertVRER-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-SARS-CoV-2-Nucleocapsid
Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD200RCD4d3+4-bio-linker-His
Plasmid#36153PurposeA positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmidDepositorInsertCD200R (Cd200r1 Rat)
UseTagsHis tag, biotinylation peptide, and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC3
Plasmid#172996Purposeconstitutive expression of FLAG-tagged WWC3DepositorInsert3xFLAG-WWC3 (WWC3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_123
Plasmid#96919Purposefor CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.DepositorInsertdCas9
UseLentiviralTagsp300ExpressionMammalianMutationdCas9PromoterEF1aAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Consensus
Plasmid#176678PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLucIPZ
Plasmid#228703PurposeConstitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX-evoCas9
Plasmid#107550PurposeExpresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cellsDepositorInsertHumanized S. pyogenes Cas9
UseCRISPRTags3XFLAGExpressionMammalianMutationM495V/Y515N/K526E/R661QPromoterCBhAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-mCherry HR donor vector
Plasmid#137928PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-mCherry-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiHiFiCas9
Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KH-RBS2-dCas9
Plasmid#182740PurposeIntegrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoterDepositorInsertdCas9
UseCRISPR; Integrative vectorTagsExpressionMutationPromoterPtetAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABECD
Plasmid#66536PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB435
Plasmid#74825PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA025
Plasmid#131384PurposeORFeus expression, mutantDepositorInsertORFeus
UseTagsORF2-3xFlagExpressionMammalianMutationH230A EnPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA034
Plasmid#131386PurposeORFeus expression, mutantDepositorInsertORFeus
UseTagsORF2-3xFlagExpressionMammalianMutationD702Y RTPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-NLucYAP15
Plasmid#107610Purposemeasure LATS activity by Luciferase AssayDepositorInsertNLucYAP15
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBK-YE1-BE3
Plasmid#85174PurposeExpresses YE1-BE3 in mammalian cellsDepositorInsertYE1-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBK-YEE-BE3
Plasmid#85177PurposeExpresses YEE-BE3 in mammalian cellsDepositorInsertYEE-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180268PurposeLentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNADepositorInsertGARP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATLIC
Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYSD300
Plasmid#212930PurposeEncodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB412
Plasmid#74806PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only
