We narrowed to 16,113 results for: form

1. Shank2 scFv [N23B/6]

   Plasmid

   #190543

   Purpose

   Mammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Shank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rufy3 scFv [N483/84]

   Plasmid

   #190559

   Purpose

   Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/84.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-SAPAP1/2 [N238/31R]

   Plasmid

   #182110

   Purpose

   Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-SAPAP1/2 () recombinant mouse monoclonal antibody
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mitoTALECD for_atp1_1397NC

   Plasmid

   #186204

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Promoter

   Arabidopsis RPS5A

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mitoTALECD for_-20G_1333CN

   Plasmid

   #186200

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mitoTALECD for_ -13G_1397CN

   Plasmid

   #186199

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mitoTALECD for_ -6G_1333CN

   Plasmid

   #186198

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-iBSD-5LO-d4

   Plasmid

   #182743

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform delta 4 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Promoter

   SFFV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LeGO-iBSD-5LO-d13

   Plasmid

   #182745

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform delta 13 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBpuro-EGFP-eDHFR(69K6)-cRaf

    Plasmid

    #178849

    Purpose

    Mammalian expression of cRaf fused to EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-cRaf (RAF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBpuro-cRaf-mNG-eDHFR(69K6)

    Plasmid

    #172107

    Purpose

    Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBoBi-hPEN2 20A

    Plasmid

    #183639

    Purpose

    Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

    Plasmid

    #180314

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 )
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    SEPT9_i1-i5

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV_SEPT9_i1 R10A R15A-msfGFP

    Plasmid

    #180336

    Purpose

    mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R10A R15A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV_SEPT9_i1 S12A S13A-msfGFP

    Plasmid

    #180337

    Purpose

    mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S12A S13A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Parvalbumin scFv [L114/81]

    Plasmid

    #182073

    Purpose

    Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAID1.2-EF1a-NmClover3-hsLem2

    Plasmid

    #179524

    Purpose

    Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mClover3-mAID

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178034

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    T7LacI

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. psiCheck Cdk2ap1ΔN (MT2B2)

    Plasmid

    #178029

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-mDrc3-PA

    Plasmid

    #176467

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 3 (Drc3 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SITS-EGFP-Bin1b

    Plasmid

    #176021

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cavin4a-mCherry

    Plasmid

    #176010

    Purpose

    Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Cavin4a (cavin4a Zebrafish)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

    Plasmid

    #175051

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
    

    Expression

    Bacterial

    Mutation

    changed Thr76 to Asp, phosphomimetic mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

    Plasmid

    #175050

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

    Plasmid

    #172200

    Purpose

    AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

    Depositor

    Garrett Kaas

    Article

    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

    Insert

    HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
    

    Use

    AAV and Mouse Targeting; Tale

    Tags

    HA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    transcription regulatory domain removed

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST17-cDAXX-0SB

    Plasmid

    #169730

    Purpose

    Bacterial expression of N-terminally 6His tagged cDAXX-0SB

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX-0SB (DAXX Human)
    

    Tags

    6 His

    Expression

    Bacterial

    Mutation

    Comprises residues 495-741 and has the major SB m…

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

    Plasmid

    #164002

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1461R

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

    Plasmid

    #164001

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W1460A

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A

    Plasmid

    #164000

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    M1457A

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A

    Plasmid

    #163999

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Y1445A

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1427E

    Plasmid

    #163998

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1427E

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1414R

    Plasmid

    #163997

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1414R

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1411R

    Plasmid

    #163996

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1411R

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1397R

    Plasmid

    #163994

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E1397R

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

    Plasmid

    #163993

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1392E

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

    Plasmid

    #163992

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R1386E

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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40. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid

    #155063

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid

    #155061

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid

    #155060

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pBOB-N-3HA-GK_v2

    Plasmid

    #134277

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    553 amino acids, isoform 2

    Promoter

    CMV

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCri-H6-TEV-GRAB-STREP

    Plasmid

    #128499

    Purpose

    Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x) and Strep tag (2x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSB61 - pL0_V2 (CDS1)

    Plasmid

    #123183

    Purpose

    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBABE (hygro) hYAP 1-2delta

    Plasmid

    #124154

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBABE (hygro) hYAP 1-1alpha

    Plasmid

    #124147

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBABE (hygro) hYAP 1-1beta

    Plasmid

    #124148

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge