We narrowed to 4,995 results for: mcs

1. hNTa1-qgRNA-pYJA5

   Plasmid

   #217779

   Purpose

   Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Δ18 B.1.1.529

   Plasmid

   #179907

   Purpose

   Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

   Insert

   SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…

   Promoter

   CAG

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

   Plasmid

   #172911

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon A122D
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

   Plasmid

   #172912

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon-ST A122D
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO-HPV33 L1L2

   Plasmid

   #52493

   Purpose

   Expresses HPV33 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV33 L1
   HPV33 L2
   

   Expression

   Mammalian

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVITRO-HPV8 L1L2

   Plasmid

   #52598

   Purpose

   Expresses HPV8 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV8 L1
   HPV8 L2
   

   Expression

   Mammalian

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVITRO-HPV38 L1L2

   Plasmid

   #52594

   Purpose

   Expresses HPV38 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV38 L1
   HPV38 L2
   

   Expression

   Mammalian

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT4/HB

   Plasmid

   #108352

   Purpose

   Optimized Sleeping Beauty Transposon Vector with MCS

   Depositor

   Wolfgang Uckert

   Article

   Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

   Type

   Empty backbone

   Use

   Transposon

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSKI

    Plasmid

    #232484

    Purpose

    pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

    Depositor

    Carlos Silva García

    Article

    Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

    Insert

    pSKI
    

    Expression

    Worm

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI108-SiT-Cas12a-[Cond]

    Plasmid

    #128407

    Purpose

    Conditionally express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-mito-tagRFP

    Plasmid

    #185599

    Purpose

    Lentiviral construct for expression of tagRFP-T targeted to mitochondria

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    mito-tagRFP-T
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

    Plasmid

    #210857

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    V44D/V314D/L324D/R543D/R548D

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-perox-tagRFP

    Plasmid

    #185600

    Purpose

    Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    perox-tagRFP-T
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

    Plasmid

    #139236

    Purpose

    mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO-HPV18 L1L2

    Plasmid

    #52592

    Purpose

    Expresses HPV18 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV18 L1
    HPV18 L2
    

    Expression

    Mammalian

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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