We narrowed to 18,334 results for: met

1. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

   Plasmid

   #69221

   Purpose

   Expresses R1333K mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Lysine

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

   Plasmid

   #69222

   Purpose

   Expresses R1333S mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Serine

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMKO-sh_lnc-BATE1

   Plasmid

   #64866

   Purpose

   Expresses shRNA targeting lnc-BATE1

   Depositor

   Lei Sun

   Article

   Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

   Insert

   shRNA targeting lnc-BATE1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   u6

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mIFP12-CAF1-C-10

   Plasmid

   #56246

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mIFP24-CAF1-C-10

   Plasmid

   #56266

   Purpose

   Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CAF1
   

   Use

   Tags

   mIFP24

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

8. TALEN-CCR5L18-NtermN3

   Plasmid

   #51448

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) N3 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K150Q, K153Q, and R154Q

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TALEN-CCR5R18-NtermN3

   Plasmid

   #51449

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) N3 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K150Q, K153Q, and R154Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFAB806

    Plasmid

    #47849

    Purpose

    BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    lambda tR2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO4

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAB805

    Plasmid

    #47850

    Purpose

    BIOFAB reporter plasmid for measuring tonB termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    tonB terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO5

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFAB816

    Plasmid

    #47851

    Purpose

    BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    ilvGEDA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO6

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFAB807

    Plasmid

    #47852

    Purpose

    BIOFAB reporter plasmid for measuring RNAI termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    RNAI terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO7

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFAB820

    Plasmid

    #47855

    Purpose

    BIOFAB reporter plasmid for measuring tetAC termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    tetAC terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO10

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFAB1910

    Plasmid

    #47859

    Purpose

    BIOFAB reporter plasmid for measuring rpoC termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rpoC terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO14

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFAB804

    Plasmid

    #47848

    Purpose

    BIOFAB reporter plasmid for measuring T3 early termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    T3 early terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO3

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAB3905

    Plasmid

    #47808

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 14 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMT-Map7-1783-end

    Plasmid

    #46071

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1783-end (Map7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMT-Map7-856-1351

    Plasmid

    #46069

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-856-1351 (Map7 Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAVf-EnhCB-lacZnls mir-122 1xBS

    Plasmid

    #35643

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    1 miR-122 target site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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