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pcDNA3.1(+)/Puro/FA/FFT-LL5BIP

Plasmid

#112830

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Publication

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LL5BIP (2410002F23Rik Mouse)

Tags

2xFlag-TEV

Expression

Mammalian

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Venus-rab11a

Plasmid

#234744

Purpose

Mammalian expression of recycling endosome marker Venus-rab11a

Depositor

Nevin Lambert

Publication

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-rab11a (RAB11A Human)

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Publication

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

qmTQ2-ATP

Expression

Mammalian

Promoter

CMV promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3654

Plasmid

#222349

Purpose

Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Publication

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA63-GG

Plasmid

#208015

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Publication

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Publication

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAB151

Plasmid

#101666

Purpose

Opto-T7RNAP*(563-F1)

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2

Expression

Mammalian

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Expression

Mammalian

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113344

Purpose

Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP64-dCas9-p300core-T2A-MS2-p65-HSF1

Use

Piggybac

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Expression

Mammalian

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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hum sgk promoter F4R4

Plasmid

#83426

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Publication

Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)

Use

Luciferase

Tags

Luciferase

Promoter

sgk

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet-H_N1

Plasmid

#85058

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MTS (COX8A Human)

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shLuc

Plasmid

#83092

Purpose

Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

Depositor

Kevin Janes

Publication

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLuc

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only
pME-eSIBR

Plasmid

#71665

Purpose

intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

Depositor

Philip Washbourne

Publication

Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

Insert

intronic eSIBR cassette

Use

RNAi; Multi-site gateway

Promoter

None

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Publication

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Publication

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Expression

Insect

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only