We narrowed to 5,107 results for: PEP

1. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

   Plasmid

   #74513

   Purpose

   AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   EGFP-p2A-EGFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD200RCD4d3+4-bio-linker-His

   Plasmid

   #36153

   Purpose

   A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200R (Cd200r1 Rat)
   

   Use

   Tags

   His tag, biotinylation peptide, and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 3xFlag IFIT5

   Plasmid

   #53556

   Purpose

   mammalian expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLAC

   Plasmid

   #45072

   Purpose

   lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

   Depositor

   Gordon Laurie

   Article

   Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

   Insert

   Lacritin (LACRT Human)
   

   Use

   Protein expression vector

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-MR1

   Plasmid

   #178603

   Purpose

   Mammalian expression of myc-tagged MR1

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-Dcp2

   Plasmid

   #153975

   Purpose

   SNAP-tag polypeptide fused to mRNA decapping enzyme 2

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA Decapping enzyme 2 (DCP2 Human)
   

   Use

   Tags

   SNAP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST26-USP47

   Plasmid

   #64326

   Purpose

   Mammalian expression and purification of USP47.

   Depositor

   Grigory Dianov

   Article

   Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

   Insert

   USP47 (USP47 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVk7-33G1-BioLHis

   Plasmid

   #58949

   Purpose

   backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   6His tag and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGG-cTP-TurboID-GFP

   Plasmid

   #209403

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   PM-3XHA-TurbOID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pStr-KDEL-IRES-ST-mCherry

    Plasmid

    #110727

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Str Streptavidin binding peptide (SBP) and mCherr…

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV and IRES

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSNAP-CLC-EYFP

    Plasmid

    #38011

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP and SNAP26m

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETcLIC-GFP

    Plasmid

    #183514

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-NPY-ClopHensor

    Plasmid

    #25939

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
    

    Use

    Tags

    NPY and Strep-II

    Expression

    Mammalian

    Mutation

    Neuropeptide Y localization signal

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pEX-CMV-SP-STIM2(15-746)

    Plasmid

    #18868

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    STIM2 (STIM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Signal peptide(SP) 1-10 from STIM1 and residues 1…

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Use

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7-LacO

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. RINS1(mut)

    Plasmid

    #107291

    Purpose

    Plasmid for the expression of the inactive mutant of RINS1 sensor

    Depositor

    Dmytro Yushchenko

    Article

    Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

    Insert

    RINS1(mut)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    two Arg/Ser mutations at the beginning and in the…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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