We narrowed to 5,421 results for: PEP

1. pAGM5355

   Plasmid

   #50296

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic) + HIS tag
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

   Plasmid

   #117983

   Purpose

   SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B2
   

   Use

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

   Plasmid

   #60903

   Purpose

   Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   Cas9 dead
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-HA-human MYCN

   Plasmid

   #74163

   Purpose

   Mammalian expression of human MycN

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MycN (MYCN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-EGFP-NR2b wt

   Plasmid

   #45447

   Depositor

   Robert Malinow, Andres Barria

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-EGFP-NR2a wt

   Plasmid

   #45445

   Depositor

   Robert Malinow, Andres Barria

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2A (Grin2a Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   dSV40 Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GFP-Dub3 mouse

    Plasmid

    #72683

    Purpose

    mammalian expression of GFP-Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos2-ER-5

    Plasmid

    #57373

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    ER (CALR Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pCMV_PACLight1

    Plasmid

    #197864

    Purpose

    Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

    Depositor

    Tommaso Patriarchi

    Article

    Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

    Insert

    PACLight1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEGFP-C1 Lifeact-EGFP-2XNLS

    Plasmid

    #58467

    Purpose

    Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    Lifeact
    2X NLS with 2X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Use

    Tags

    Cre

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

18. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-SpyTag002-MBP

    Plasmid

    #102831

    Purpose

    Bacterial expression of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to the N-terminus of MBP via a …

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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