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pKT-CNP

Plasmid

#211803

Purpose

Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

Depositor

Emily Weinert

Publication

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Promoter

tet

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAV hSyn hPGK1-mRuby

Plasmid

#220911

Purpose

Neuronal specific expression of the fusion construct PGK1-mRuby

Depositor

Timothy Ryan

Publication

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1-mRuby (PGK1 Human)

Use

AAV

Tags

mRuby

Expression

Mammalian

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Publication

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Publication

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Publication

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Publication

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Publication

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

TVA (CD320 Chicken)
TurboRFP

Expression

Mammalian

Promoter

sCMV and sCMV IE94

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13

Plasmid

#157712

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp13 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp10

Plasmid

#157706

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nsp10 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPPAT-2XStrep

Plasmid

#125620

Purpose

expresses human PPAT protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Publication

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

PPAT (PPAT Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shQKI-3371

Plasmid

#115455

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3371 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Publication

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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RECQL5 (RECQL5A-c011)

Plasmid

#98231

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RECQL5A (RECQL5 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

Promoter

T7

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Publication

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-GP-2-CBX1 Chromo

Plasmid

#59697

Purpose

Contains histone modification interacting domain (CBX1 Chromo)

Depositor

Albert Jeltsch

Publication

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX1 Chromo (CBX1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Publication

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-E192C

Plasmid

#180233

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

Depositor

Joanna Slusky

Publication

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domains (residue…

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSL1180-NiV L(2k)-mCherry/eGFP

Plasmid

#234654

Purpose

Minigenome plasmid of NiV Bangladesh strain

Depositor

Carolina Lopez

Publication

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

part L gene
mCherry
eGFP

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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