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Showing: 361 - 380 of 471 results

  5. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  6. TFORF1683

    Plasmid
    #142826
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TWIST1 (TWIST1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    142826-plasmid-map-sequence-id-392723

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  8. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid
    #154774
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154774-plasmid-map-sequence-id-310423

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  9. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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  16. pcDNA5/FRT/TO/SH/GW-Mst2

    Plasmid
    #84300
    Purpose
    Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.
    Depositor
    Jonathan Chernoff
    Article
    Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71
    Insert
    Mst2 (STK3 Human)
    Use
    Tags
    HA, SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

    Plasmid
    #226578
    Purpose
    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
    Depositor
    Gia Voeltz
    Article
    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382
    Insert
    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    226578-plasmid-map-sequence-id-451909

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Showing: 361 - 380 of 471 results