We narrowed to 21,801 results for: Sis

1. pRK-flag-GFP 1-10 tetra Cys

   Plasmid

   #78589

   Purpose

   Expression flag-GFP 1-10 tetra Cys in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-GFP 1-10 tetra Cys
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLKO.1-mPRAS40

   Plasmid

   #15480

   Depositor

   Do-Hyung Kim

   Article

   Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

   Insert

   PRAS40 shRNA (Akt1s1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX544 SpCas9(del1098-1368)

   Plasmid

   #58889

   Purpose

   Truncated form of SpCas9 without C' domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 1098-1368

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCitrine-USP19 1-1290

   Plasmid

   #78595

   Purpose

   Express mCitrine-USP19 1-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   USP19 1-1290 (USP19 Human)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

5. PX543 SpCas9(del175-307)

   Plasmid

   #58888

   Purpose

   Truncated form of SpCas9 without REC2 domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 175-307

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-Cas9

   Plasmid

   #120352

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL2082

   Plasmid

   #155162

   Purpose

   Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 248-269 in MlaF

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEL1514

   Plasmid

   #155147

   Purpose

   Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL1965

    Plasmid

    #155152

    Purpose

    Expresses MlaF(1-246)-(6 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1966

    Plasmid

    #155153

    Purpose

    Expresses MlaF(1-246)-(10 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1967

    Plasmid

    #155154

    Purpose

    Expresses MlaF(1-246)-(14 aa linker)-GCN4

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    mlaF(1-246)-GCN4_Dimerization_Domain
    

    Use

    Tags

    Expression

    Mutation

    deleted amino acids 247-269 in MlaF; added dimeri…

    Promoter

    araBAD

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAG-Avr-HPRT-RIGHT

    Plasmid

    #107272

    Purpose

    Expresses TALEN (right) targeting HPRT exon3

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    HPRT1_B Avr-TALEN (right)
    

    Use

    TALEN

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAG-Avr-HPRT-LEFT

    Plasmid

    #107271

    Purpose

    Expresses TALEN (left) targeting HPRT exon3

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    HPRT1_B Avr-TALEN (left)
    

    Use

    TALEN

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-EFS-PEmax-P2A-Puro

    Plasmid

    #228890

    Purpose

    Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Inserts

    PEmax
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge