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Showing: 721 - 760 of 847 results

  1. pOCC6

    Plasmid
    #118864
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, EGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118864-plasmid-map-sequence-id-232291

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  2. pOCC16

    Plasmid
    #118870
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    Use
    Tags
    EGFP and HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118870-plasmid-map-sequence-id-232333

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  4. pME_G_0_5_001_3'UTR_Placeholder

    Plasmid
    #235905
    Purpose
    Encodes a sfGFP cassette in the 3'UTR position used for placeholder cloning
    Depositor
    Tobias Erb
    Insert
    3'UTR placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235905-plasmid-map-sequence-id-471486

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  5. pME_G_0_4_001_CDS_Placeholder

    Plasmid
    #235892
    Purpose
    Encodes a sfGFP cassette in the CDS position used for placeholder cloning
    Depositor
    Tobias Erb
    Insert
    CDS placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235892-plasmid-map-sequence-id-471478

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  6. pME_G_0_2_001_Promoter_placeholder

    Plasmid
    #235808
    Purpose
    Encodes a sfGFP cassette in the promoter position used for placeholder cloning
    Depositor
    Tobias Erb
    Insert
    Promoter placeholder
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235808-plasmid-map-sequence-id-471433

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  8. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  9. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  10. CMV+ episome

    Plasmid
    #169742
    Purpose
    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169742-plasmid-map-sequence-id-334220

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  11. PGK+ episome

    Plasmid
    #170041
    Purpose
    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    170041-plasmid-map-sequence-id-334558

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  12. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  14. MTK0_047

    Plasmid
    #123977
    Purpose
    Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hCLYBL CAS9 Destination - HygroR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123977-plasmid-map-sequence-id-264211

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  17. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  19. MTK0_004

    Plasmid
    #123961
    Purpose
    Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    cRosa26 destination
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    123961-plasmid-map-sequence-id-272155

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  20. MTK234_056

    Plasmid
    #123921
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-hu6_20N_cr3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123921-plasmid-map-sequence-id-250076

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  21. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

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  23. pSelAct-Express

    Plasmid
    #174375
    Purpose
    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Expression vector for targeted integration
    Tags
    Expression
    Mutation
    Promoter
    pCMP1
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    174375-plasmid-map-sequence-id-370623

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  25. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224898-plasmid-map-sequence-id-443622

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  33. pLM1110_LEU2

    Plasmid
    #168460
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    mRFP1
    Use
    Synthetic Biology; Yeast assembly vector for big-…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168460-plasmid-map-sequence-id-365349

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  35. pRB051_LEU2

    Plasmid
    #196993
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196993-plasmid-map-sequence-id-394871

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  37. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid
    #89890
    Purpose
    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    HA-BirA-2A-citrine
    Use
    Unspecified
    Tags
    Citrine
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89890-plasmid-map-sequence-id-181309

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  38. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  39. MTK0_046

    Plasmid
    #123976
    Purpose
    Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hThal5.10-2_hg18 CAS9 Destination - HygroR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123976-plasmid-map-sequence-id-251798

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Showing: 721 - 760 of 847 results