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We narrowed to 578 results for: SPY

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Showing: 361 - 380 of 578 results

  3. pSC-LPETG

    Plasmid
    #207629
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-LPETG
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207629-plasmid-map-sequence-id-409853

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  4. InterTag-1xL

    Plasmid
    #186911
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Article
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    SpyTag003-sfCherry11-(GGGGS)1-PDGFR
    Expression
    Mammalian
    Available Since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186911-plasmid-map-sequence-id-369406

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  5. pYPQ262m3

    Plasmid
    #149556
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149556-plasmid-map-sequence-id-306141

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  6. pSC-cUnaG-LPETG

    Plasmid
    #207635
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207635-plasmid-map-sequence-id-409865

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  7. pYPQ265H

    Plasmid
    #149554
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    hAID-ziSpyMacCas9(D10A)-UGI
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149554-plasmid-map-sequence-id-308763

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  8. pYPQ266B

    Plasmid
    #149555
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ziSpyMacCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domai…
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149555-plasmid-map-sequence-id-306140

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  11. PB-PE iSM_PPL2

    Plasmid
    #235996
    Purpose
    Expresses iSM_PPL2 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    iSM_PPL2_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    replacement of 5 amino acids of Smac-Cas9 PL2 wit…
    Available Since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235996-plasmid-map-sequence-id-470115

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  12. SUMO-KTag

    Plasmid
    #51723
    Purpose
    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag
    Depositor
    Mark Howarth
    Article
    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.
    Insert
    SUMO-KTag
    Tags
    His6 Tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pDEST14-DoubleCatcher-H-Lock

    Plasmid
    #216285
    Purpose
    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher H-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216285-plasmid-map-sequence-id-433331

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  16. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid
    #216286
    Purpose
    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ⍺-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216286-plasmid-map-sequence-id-433335

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  17. pDEST14-DoubleCatcher-β-Lock

    Plasmid
    #216287
    Purpose
    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher β-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216287-plasmid-map-sequence-id-433337

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  18. pDEST14-DoubleCatcher-γ-Lock

    Plasmid
    #216288
    Purpose
    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher γ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216288-plasmid-map-sequence-id-433338

    Enlarge

  19. pDEST14-DoubleCatcher-δ-Lock

    Plasmid
    #216289
    Purpose
    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher δ-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216289-plasmid-map-sequence-id-433340

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  20. pDEST14-DoubleCatcher-ε-Lock

    Plasmid
    #216290
    Purpose
    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher ε-Lock
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216290-plasmid-map-sequence-id-433359

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Showing: 361 - 380 of 578 results