We narrowed to 145,976 results for: ins;

1. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172504

   Purpose

   Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

   Plasmid

   #171375

   Purpose

   For the mammalian expression of rat Gαi3 internally tagged with YFP

   Depositor

   Mikel Garcia-Marcos

   Article

   Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

   Insert

   Gai3-YFP
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

   Plasmid

   #229819

   Purpose

   A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-SARS-CoV-2 Spike-deltaC18 InsertionR214-EPE

   Plasmid

   #212461

   Purpose

   Encodes SARS-CoV-2 spike R214 insertion for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike R214 insertion (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R214 insertion

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

   Plasmid

   #193678

   Purpose

   Constitutive retroviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Deletion of SET and Zinc finger domains

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

   Plasmid

   #91819

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. M61 pPET28c-Xenopus beta catenin domains R10C

   Plasmid

   #17202

   Depositor

   Randall Moon

   Insert

   beta catenin (ctnnb1.L Frog)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   domains R10C

   Promoter

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

8. M60 pPET28a-Xenopus beta catenin domains NR7

   Plasmid

   #17201

   Depositor

   Randall Moon

   Insert

   beta catenin (ctnnb1.L Frog)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   domains NR7

   Promoter

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEM376 - sgRNA - spc4 - array - insertion - unc-119 - locus

    Plasmid

    #182344

    Purpose

    sgRNA4 for insertion of extrachromosomal arrays into the ce-unc-119 genomic locus. Use with pSEM371 fragment included in Ex array.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Spacer 4
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine

    Plasmid

    #197680

    Purpose

    "Insert(high)-pEF1s(high)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine

    Plasmid

    #197682

    Purpose

    "Insert(low)-pEF1s(low)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

    Plasmid

    #197681

    Purpose

    "Insert(high)-pEF1s(orig)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

    Plasmid

    #197683

    Purpose

    "Insert(low)-pEF1s(orig)" reporter plasmid

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    pEF1s-H2B-Citrine
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

    Plasmid

    #180811

    Purpose

    Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BTLA (Y257F, Y282F)

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid

    #91820

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Plasmid

    #180806

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BTLA (Y243F, Y257F, Y282F)

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid

    #180807

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y257F, Y282F)

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge