We narrowed to 148,387 results for: ins;

1. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM320 - sgRNA mosTI unc-119 array insertion

   Plasmid

   #159824

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 array insertion
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115213

   Purpose

   Expresses RanCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   RanCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115218

   Purpose

   Expresses PguCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PguCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172505

   Purpose

   Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Plasmid

   #58440

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
   

   Expression

   Yeast

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Plasmid

   #58439

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
   

   Expression

   Yeast

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. p255 pPr-CREM/Ins/CMV-STOP-luc

   Plasmid

   #8408

   Purpose

   Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   CREM/insulators/lox/luciferase
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   Probasin, CMV

   Available Since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

9. FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Plasmid

   #58441

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
   

   Expression

   Yeast

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. M59 pPET28a-TEV-human beta cat domains R1-C

    Plasmid

    #17200

    Depositor

    Randall Moon

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains R1C

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

    Plasmid

    #171375

    Purpose

    For the mammalian expression of rat Gαi3 internally tagged with YFP

    Depositor

    Mikel Garcia-Marcos

    Article

    Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

    Insert

    Gai3-YFP
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV-2 Spike-deltaC18 InsertionR214-EPE

    Plasmid

    #212461

    Purpose

    Encodes SARS-CoV-2 spike R214 insertion for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike R214 insertion (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    R214 insertion

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

    Plasmid

    #193678

    Purpose

    Constitutive retroviral expression of PRDM14

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Retroviral

    Mutation

    Deletion of SET and Zinc finger domains

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. M61 pPET28c-Xenopus beta catenin domains R10C

    Plasmid

    #17202

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains R10C

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

18. M60 pPET28a-Xenopus beta catenin domains NR7

    Plasmid

    #17201

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains NR7

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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