We narrowed to 610 results for: prime editing
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Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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fluoPEER-GFP>BFP
Plasmid#185477PurposeFluorescent reporter for a Y66H mutation to convert a GFP to BFP.DepositorInsertGFP-Y66-editing-site
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
fluoPEER-HEK3_5bp-del
Plasmid#185476PurposeFluorescent reporter for a 5 basepair deletion at the HEK3 locus.DepositorInsertHEK3 editing site
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*-3HA
Plasmid#169854PurposeMammalian Expression, SpyCas9 PE2* prime editor with 3HA-tagDepositorInsertSpCas9-H840A-star-M-MLV-3HA
Tags3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
QPM-sgR (pTaU3)
Plasmid#140448PurposeFor sgRNA expression in monocotyledons protoplastsDepositorInsertTaU3-sgRNA
UseCRISPRExpressionPlantPromoterTaU3Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-eGFP
Plasmid#191104PurposeLentiviral expression plasmid of hMLH1dn with P2A-eGFP reporterDepositorInserthMLH1dn
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pp-CBBC-Fluc
Plasmid#232920PurposeAllows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_mScarlet-I_Donor
Plasmid#173207PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N mScarlet-I donor
UseCRISPRExpressionMammalianPromoterhPGK1Available SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6nH1-HD10kb
Plasmid#172657PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD10kbA/pegRNA-HD10kbB
UseCRISPRAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_SRD5A1-mcherry
Plasmid#159780PurposeS. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 geneDepositorInsertspacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lentiguide-Blast-eNMU710
Plasmid#172658PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locusDepositorInsertpegRNA-eNMUA/pegRNA-eNMUB
UseCRISPRAvailable SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-HD252
Plasmid#172656PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD252A/pegRNA-HD252B
UseCRISPRAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT159_AAV_p3_PE7-C_hU6_epegRNA
Plasmid#251105PurposeEncodes the C terminal of the PE7 prime editor in an AAV expression plasmid + pegRNA cloning casetteDepositorInsertPE7 C-terminal
UseAAV and CRISPRPromoterp3Available SinceMay 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hyPE2
Plasmid#177173PurposeExpresses hyPE2 in mammalian cellsDepositorInserthyperPE2
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PE2-C
Plasmid#164908PurposeExpresses the C-terminal split-intein fragment of PE2DepositorInsertPE2-C
UseAAVAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PE2-N
Plasmid#164909PurposeExpresses the N-terminal split-intein fragment of PE2DepositorInsertPE2-N
UseAAVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…ExpressionPlantMutationH840A, R240K, N413K,Available SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only