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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 418 results for: px458

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Showing: 361 - 380 of 418 results

  1. pKMW281_dSpRY

    Plasmid
    #244822
    Purpose
    Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)
    Depositor
    Jennifer Doudna
    Publication
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Insert
    dSpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CAG
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244822-plasmid-map-sequence-id-486583

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  5. pSCIP-hTRAC

    Plasmid
    #154094
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting human TRAC locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154094-plasmid-map-sequence-id-307107

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  6. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  9. pKMW123_SpRY-Cas9_U6-entry

    Plasmid
    #244820
    Purpose
    Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette
    Depositor
    Jennifer Doudna
    Publication
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…
    Promoter
    CAG, U6
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244820-plasmid-map-sequence-id-486582

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Showing: 361 - 380 of 418 results