We narrowed to 866 results for: seph

1. human a5 full length

   Plasmid

   #217818

   Purpose

   Full length human integrin a5 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGA5 (ITGA5 Human)
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATX3-D1

   Plasmid

   #184246

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
   

   Use

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon N263X

   Promoter

   T7 promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

   Plasmid

   #100716

   Purpose

   B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting ATP1A1 (cloned using BsmBI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis-MBP-BrCas12b-RFND

   Plasmid

   #195339

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis and 6xHis, MBP, TEV cleavage site

   Expression

   Bacterial

   Mutation

   R160E, F208W, N524V, D868V

   Promoter

   T7

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 6xHis-MBP-BrCas12b-FNLDTA

   Plasmid

   #195340

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis Tag and MBP, TEV cleavage site, 6x His Tag

   Expression

   Bacterial

   Mutation

   F208W, N524V, L795I, D868V, T874S, A1015E

   Promoter

   T7 Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. B52 + CHEK2 sgSTOP

   Plasmid

   #100712

   Purpose

   B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B52 + FANCM sgSTOP

   Plasmid

   #100710

   Purpose

   B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

   Plasmid

   #102277

   Purpose

   For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)

   Depositor

   Samie Jaffrey

   Article

   Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.

   Insert

   YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
   

   Use

   For mammalian cell-free protein expression system…

   Tags

   GST, HA, and HIS

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1A-CD19.28H.28TM.BB.3z

   Plasmid

   #200679

   Purpose

   Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-EF1A-CD19.28H.28TM.28.3z

    Plasmid

    #200678

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-EF1A-CD19.8H.8TM.BB.3z

    Plasmid

    #200671

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-EF1A-CD19.28H.28TM.28.BB.3z

    Plasmid

    #200680

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-EF1A-CD19.8h.8TM.28.3z

    Plasmid

    #200670

    Purpose

    Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-EF1A-CD19.28H.28TM.BB.28.3z

    Plasmid

    #200681

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-EF1A-CD19.8H.28TM.28.3z

    Plasmid

    #200674

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-EF1A-CD19.8H.8TM.28.BB.3z

    Plasmid

    #200672

    Purpose

    Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-EF1A-CD19.8H.28TM.BB.3z

    Plasmid

    #200675

    Purpose

    Modular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1A-CD19.8H.8TM.BB.28.3z

    Plasmid

    #200673

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid

    #200677

    Purpose

    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid

    #200676

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge