We narrowed to 30,419 results for: uros

1. pLCKO_EIF3D_sgRNA_4

   Plasmid

   #74188

   Purpose

   lentiviral vector expressing sgRNA targeting EIF3D

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   EIF3D sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX459_HR_Prnp_3

   Plasmid

   #78623

   Purpose

   pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   HR_Prnp_3 (Prnp Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV asyn S87E

   Plasmid

   #36057

   Depositor

   Hilal Lashuel

   Article

   Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

   Insert

   alpha-synuclein S87E (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S87E

   Promoter

   CMV

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

4. 49535-sgFMR1_E3B

   Plasmid

   #157783

   Purpose

   Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

   Insert

   FMR1 (FMR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Gr4-T2A-QF2 HDR plasmid

   Plasmid

   #140944

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLCKO_PSMB2_sgRNA_1

   Plasmid

   #74184

   Purpose

   lentiviral vector expressing sgRNA targeting PSMB2

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMB2 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PR594-His-bio

   Plasmid

   #52332

   Purpose

   Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PR594 (NSG1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FB002

   Plasmid

   #119676

   Purpose

   Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator tub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Kv7.4 (1-645, Δ368-523)/pcDNA3.1

   Plasmid

   #111456

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523), C643A

   Promoter

   Available since

   May 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLCKO_PSMB2_sgRNA_5

    Plasmid

    #74185

    Purpose

    lentiviral vector expressing sgRNA targeting PSMB2

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMB2 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZac2.1-GfaABC1D-mCherry-hPMCA2x/b

    Plasmid

    #111569

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-PIG-FLAG-TAF4(827-909)

    Plasmid

    #105617

    Purpose

    retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-chCARex

    Plasmid

    #87233

    Purpose

    bacterial expression of GST-CAR extracellular domain

    Depositor

    Fritz Rathjen

    Article

    Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-CMV-cGASΔN C396/7A-HA

    Plasmid

    #130918

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN C396/7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX61

    Plasmid

    #46852

    Purpose

    TOP Hupki-puro2A-Hupki-wt plasmid

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sg_Brd9_i5-ipUSEPR-TR657

    Plasmid

    #228922

    Purpose

    Knockdown of Brd9 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Brd9_i5 (Brd9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

    Plasmid

    #214889

    Purpose

    Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

    Insert

    GtACR2_mCerulean_bHK_Chrimson
    

    Use

    Tags

    mCerulean

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAB17 (punc-17::QuasAr)

    Plasmid

    #214888

    Purpose

    Expresses the voltage sensor QuasAr2 in cholinergic neurons of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

    Insert

    QuasAr2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

    Plasmid

    #214891

    Purpose

    Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

    Insert

    GtACR2_mCerulean_bHK_Chrimson
    

    Use

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233157

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMXs-mNgn2x3HA-P2A-mRuby2

    Plasmid

    #233163

    Purpose

    Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

    Insert

    mNgn2x3HA-P2A-mRuby2 (Neurog2 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    x3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Flag-mproSAAS (27 kDa)

    Plasmid

    #231635

    Purpose

    Mammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.

    Depositor

    Iris Lindberg

    Article

    Peinado et al ACS Chem Neurosci. 2022 Jun 1;13(11):165

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. sg_Brd9_i2-ipUSEPR-TR657

    Plasmid

    #228921

    Purpose

    Knockdown of Brd9 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Brd9_i2 (Brd9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RNF149 shRNA-TRE

    Plasmid

    #225337

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF149 shRNA (Rnf149 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (tetracycline-responsive element)

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid

    #221447

    Purpose

    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    SPR degron: hGEM (1-110) fragment that is fused t…

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. plenti hSyn AldoC

    Plasmid

    #220917

    Purpose

    Neuronal specific expression of AldoC

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    AldoC (ALDOC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. plenti hSyn HK1

    Plasmid

    #220915

    Purpose

    Neuronal specific expression of HK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    HK1 (HK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

    Plasmid

    #216276

    Purpose

    AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Expression

    Mutation

    Promoter

    human synapsin 1 (hSyn)

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. dCas9-RPT6-FLAG

    Plasmid

    #205416

    Purpose

    Expresses dCas9-RPT6 with FLAG label

    Depositor

    Timothy Jarome

    Article

    Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

    Insert

    dCas9-RPT6 (Psmc5 Rat)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. lentiCRISPR v2-sgWAVE2-2

    Plasmid

    #210136

    Purpose

    knock out WAVE2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Actin-binding protein WASF2 (WASF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lentiCRISPR v2-sgWAVE2-1

    Plasmid

    #210135

    Purpose

    knock out WAVE2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Actin-binding protein WASF2 (WASF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Cav2.2 pCAGGS

    Plasmid

    #206101

    Purpose

    expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHW539

    Plasmid

    #198811

    Purpose

    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    G310D mutation to abolish the binding of cAMP of …

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

    Plasmid

    #195552

    Purpose

    Expresses Thbs1 under control of short GFAP promoter

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    short GFAP

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV(MinDis)-iEscSnFR_PM

    Plasmid

    #182813

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_PM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV(MinDis)-iEscSnFR_cyto

    Plasmid

    #182814

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_cyto
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge