We narrowed to 30,684 results for: PLE
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Plasmid#165774PurposeMultiple cloning site part for enhancer cloning, requires minimal promoter elementDepositorInsertMCS-TCCC
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL12-decoder
Plasmid#159447PurposeInsert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.DepositorInsertPlexAtet-Citrine-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
TagsmCherryExpressionMammalianPromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA eGFP MALAT1
Plasmid#132643PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that ends in the MALAT1 triple helixDepositorInserteGFP
ExpressionInsectPromoterMtnAAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1211
Plasmid#129521PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFlag-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW218
Plasmid#113240PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flipDepositorInsertsCas1
Cas2
Leader-repeat
ExpressionBacterialMutationAAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…Available SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc-mCE
Plasmid#82470PurposeExpression of myc epitope and murine mRNA Capping Enzyme in mammalian cellsDepositorAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1P-natMX6-pcr2P
Plasmid#80620Purposeto delete part of the mat1-P locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-P
PCR2 from mat1-P
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-pcr1M-natMX6-pcr2M
Plasmid#80621Purposeto delete part of the mat1-M locus and fix the mating type in natural S. pombe isolatesDepositorInsertsPCR1 from mat1-M
PCR2 from mat1-M
ExpressionYeastAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
SAGA005
Plasmid#240754PurposePlasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc
UseSynthetic BiologyMutationMutation on translation initiation region of tetAAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226291PurposeLentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInserts2xMyc-tdMCP-SNAPtag
EGFP-IRES-BlastR
UseLentiviralTags2xMyc-tdMCPExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNA-Protein Landing Pad
Plasmid#226162PurposeUsed to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expressionDepositorInsertsmTagBFP2-P2A-iCasp9
Reverse tetracycline transactivator 3
Hygromycin-IRES-EGFP
UseSynthetic BiologyTagsHAExpressionMammalianAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only