We narrowed to 17,568 results for: bon;
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Plasmid#196420PurposeEmpty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorTypeEmpty backboneUseAAV and Cre/Lox; Astrocyte-selectiveExpressionMammalianPromoterCAGAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
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pGMC00028 (aka pTS0057)
Plasmid#200832PurposeAAVS1 guide RNA cloned into the lentiSAM puro backboneDepositorInsertAAVS1 guide RNA
UseLentiviralExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA(lib)-puro
Plasmid#119976PurposeLenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT2-Flag
Plasmid#102623PurposeExpresses full length SIRT2 isoform 1 in mammalian cellsDepositorAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
ExpressionBacterial and MammalianPromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-egfp-U3-Osp.gRNA
Plasmid#166034PurposeEmpty backbone for expression of gRNA-expressing integration deficient lentiviral particles.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cre-responsive Cas9 expressing transposon library vector
Plasmid#164138PurposeBackbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and Synthetic BiologyExpressionMammalianPromoterhuman U6, EF-1aAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF
Plasmid#51076Purposemammalian expression of wt ARAFDepositorAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPS
Plasmid#85801PurposeBackbone for pooled cloning of Perturb-seq librariesDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIO-DSE-mCherry-PSE-MCS
Plasmid#129669PurposeBackbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressedDepositorTypeEmpty backboneUseAAVTagsmCherryPromoterEF1aAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161925PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2-V5
Plasmid#180413Purposelentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI
Plasmid#226009PurposeDjCas13d guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
TagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianPromoterCMVAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9-KKH-Puro
Plasmid#135966PurposeExpresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUHD.NFATc1nuc
Plasmid#40616DepositorInsertNFATc1nuc (NFATC1 Human)
TagsHAExpressionMammalianMutationS172A S175A S176A S178A S179A S181A S184A S187A S…PromoterCMV2Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR
Plasmid#37276PurposeTALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)DepositorTypeEmpty backboneTagsC terminal Tal From pTAL3, FokI RR, HA, and N-ter…ExpressionBacterial and MammalianPromoterCMVAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM3-Flag
Plasmid#180408Purposelentiviral transduction of human TGM3 (with a C-terminal Flag tag) geneDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only