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pDONR223_KEAP1_p.G333C

Plasmid

#82835

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

G333C; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.G31A

Plasmid

#81524

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway Cloning

Mutation

G31A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.E17K

Plasmid

#81459

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway Cloning

Mutation

E17K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p{CFD4-EYFP-3xP3::DsRed}

Plasmid

#86863

Purpose

Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Expression

Insect

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

Plasmid

#74261

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pGEX NBP1 (P#415)

Plasmid

#14658

Depositor

Matija Peterlin

Article

Lu et al Immunity. 1998 May . 8(5):647-56.

Insert

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pT7CFE1-TelR15-sfGFP

Plasmid

#49645

Purpose

TALEGreen-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

sfGFP

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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ratGLUT2

Plasmid

#14946

Depositor

Bernard Thorens

Article

Thorens et al J Biol Chem. 1996 Apr 5. 271(14):8075-81

Insert

GLUT2 (Slc2a2 Rat)

Expression

Mammalian

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3_CDC50A

Plasmid

#203694

Purpose

expresses CDC50A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Mouse, Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Promoter

LTR

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-VPS15

Plasmid

#99326

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

VPS15 (PIK3R4 Human)

Tags

Strep-Strep-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pFA6a-HTB-kanMX6

Plasmid

#26875

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂PY)

Plasmid

#24808

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the PY motif: PPPY

Available Since

June 22, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pTXB1-EXO1b

Plasmid

#68267

Purpose

Bacterial expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
p-AAV2-hSyn-MerMAID6-Citrine

Plasmid

#126521

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID6

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-iSAM

Plasmid

#211495

Purpose

AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

UniSAM insert
TET-ON
Neo Resistance
Insulator

Use

CRISPR and Synthetic Biology

Tags

T2A-mCherry Tag

Expression

Mammalian

Promoter

EF1a, NA, Splicing acceptor, and TREGV promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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