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pYFAC-CH4

Plasmid

#168980

Purpose

AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in asp…

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EYFP Peroxisome visual barcode

Plasmid

#158677

Purpose

tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jRex-GECO

Plasmid

#169259

Purpose

AAV transfer plasmid for neuronal expression of jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

JRex-GECO

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH2

Plasmid

#168978

Purpose

AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in asp…

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

Plasmid

#62896

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAT-WT-HA-GFP11

Plasmid

#177719

Purpose

ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70

Plasmid

#62912

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_mTurquoise2

Plasmid

#113895

Purpose

Mammalian (inducible) expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Turquoise2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR

Plasmid

#217657

Purpose

Expresses norepinephrine indicator nLightR under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-RiboL1-jGCaMP8f

Plasmid

#192612

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Use

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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acGFP WC visual barcode

Plasmid

#158672

Purpose

tagging clonal populations with whole cell acGFP fluorescent protein

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes6

Plasmid

#15356

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

hIes6 (INO80C Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only