We narrowed to 44,096 results for: cha

1. RcSxph D794E

   Plasmid

   #194041

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794E

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RcSxph D794N

   Plasmid

   #194042

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   D794N

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RcSxph Y795A

   Plasmid

   #194043

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y795A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RcSxph Y558A/I782A

   Plasmid

   #194044

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y558A/I782A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2-HsProm1s1-TwinStrep-His

   Plasmid

   #210816

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2-HsProm1s1-StayGold

   Plasmid

   #210821

   Purpose

   Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   StayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2H12ref

   Plasmid

   #221188

   Purpose

   cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   None (same as cdGreen) and Ptet

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWR88

   Plasmid

   #203422

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, no genomic target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   CAR copy 1
   P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
   CAR copy 2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWR23

   Plasmid

   #202770

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coKanMX
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

    Plasmid

    #207024

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

    Plasmid

    #207023

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

    Plasmid

    #207022

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

    Plasmid

    #207020

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

    Plasmid

    #207019

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMCSG7-10xHis-ecRNHI*

    Plasmid

    #219523

    Purpose

    bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

    Depositor

    Arthur Palmer

    Article

    Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

    Insert

    cysteine-free variant of the E. coli ribonuclease HI gene
    

    Use

    Tags

    N terminal 10xHis Tag

    Expression

    Bacterial

    Mutation

    Cysteine free variant

    Promoter

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Hyg-BFP-P2A-ppViperin

    Plasmid

    #217481

    Purpose

    Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP-P2A-otPKRFL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

    Plasmid

    #179717

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMS110

    Plasmid

    #215674

    Purpose

    Split hygromycinR landing pad insertion plasmid for ChrIII

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    3' ∆HYGR is promoterless and encodes aa60-341

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NFAST(low)-Halo-ER

    Plasmid

    #214419

    Purpose

    ER localization of low-affinity NFAST fused to Halo-ER

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET SUMO-syt1 (96-421)

    Plasmid

    #213619

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET SUMO-syt1 (96-421) I367C

    Plasmid

    #213620

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) I367C (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    C277A; I367C

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET SUMO-syt7 (136-403)

    Plasmid

    #213621

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 (136-403)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 (136-403) (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET SUMO-syt1 5W

    Plasmid

    #213623

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 5W

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 5W (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    M173W, F231W, F234W, C277A, V304W, Y364W, I367C

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET SUMO-syt1 s7+

    Plasmid

    #213624

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 s7+

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 s7+ (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET SUMO-syt7 C2AB - 4A

    Plasmid

    #213625

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB - 4A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, I298A, L361A

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET SUMO-syt7 C2A-2A 2B

    Plasmid

    #213626

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2A-2A 2B (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, R363H

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET SUMO-syt7 C2AB-2A

    Plasmid

    #213627

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB-2A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    I298, L361A

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET SUMO-FL-syt1 5W

    Plasmid

    #213629

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1 5W

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 5W (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    M173W, F231W, F234W, C277A, V304W, Y364W, I367C

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    Promoter

    pATG8

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBabe-Sik-YF

    Plasmid

    #210723

    Purpose

    Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28-hXRCC1pd-HIS

    Plasmid

    #206036

    Purpose

    Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    T7

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pWR54

    Plasmid

    #203330

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209011

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX SYT9 C2A D197,199N

    Plasmid

    #195704

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 124-211 of mouse SYT9 with D1…

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pWR24

    Plasmid

    #202771

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coNAT
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWR16

    Plasmid

    #202711

    Purpose

    integrating bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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41. pFsynW SYT1deltaC2AB reporter

    Plasmid

    #197279

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. pJL1-MBP-DQNAT

    Plasmid

    #198215

    Purpose

    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    MBP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pJL1-PD-DQNAT

    Plasmid

    #198216

    Purpose

    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    PD-DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pMC-6-AmCyan-P2A-NptII-9

    Plasmid

    #197742

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AmCyan-P2A-NptII bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. CapChR1_pUAST-attb

    Plasmid

    #188040

    Purpose

    Expresses CapChR1 in Drosophila

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1
    

    Use

    Tags

    none

    Expression

    Insect

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    UAS

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pETM-41P-HsXRN1_1174-1649-Strep_AH

    Plasmid

    #148901

    Purpose

    Bacterial Expression of HsXRN1_1174-1649

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

    Insert

    HsXRN1_1174-1649 (XRN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

    Plasmid

    #167899

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

    Plasmid

    #167897

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

49. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

    Plasmid

    #167903

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

50. pIALD2S

    Plasmid

    #185865

    Purpose

    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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