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pCMV_Anc689-nCas9

Plasmid

#163526

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191155

Purpose

Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:DNAJB1.s1:EGFP-3xMS2

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRc/CMV-Beta(4)Integrin

Plasmid

#16265

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

lacked the 70- and 7-amino acid splice variants a…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac-His6-Mps1-WT

Plasmid

#114063

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-WT (TTK Human)

Tags

His6

Expression

Insect

Mutation

none

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-nCas9

Plasmid

#163531

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Expression

Bacterial

Promoter

lac promoter

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_EE-nCas9

Plasmid

#163541

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

EE-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct11

Plasmid

#28204

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa193-274 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Beta(4)Integrin-DeltaCYT

Plasmid

#16264

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

truncated cytoplasmic tail introduced a stop cod…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pTox-EGFPsite1_NGG-PAM (BPK740)

Plasmid

#181746

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)

Use

Selection plasmid

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p4979 pcDNA4-E6AP III L502P

Plasmid

#32892

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

L502P

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
p4978 pcDNA4-E6AP III E550L

Plasmid

#32891

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

E550L

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
tdp43-EGFP construct10

Plasmid

#28203

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-105 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pMiR-E6AP-3'UTR

Plasmid

#53694

Purpose

Luciferase reporter assay for microRNA binding on E6AP 3'UTR

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only