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Plasmid#194292PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJDepositorInsertcavin1b (cavin1b Zebrafish)
UseMultisite gateway entry vectorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-lox
Plasmid#190059PurposeExpress the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertlox
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-codA
Plasmid#190058PurposeExpress the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertcodA
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pYjjZ-RBS- sfGFP
Plasmid#190054PurposeGFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pKatG-RBS- sfGFP
Plasmid#190053PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pOxyS-RBS- sfGFP
Plasmid#190052PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Full-length P229S talin1
Plasmid#182993Purposeexpress FL variant P229S talin1 in mammalian cellsDepositorAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mini-talin P229S (headR13DD P229S)
Plasmid#182995Purposeexpression of minitalin P229S in mammalian cellsDepositorAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
talin1-head P229S
Plasmid#182997Purposeexpression of talin1 head P229S in mammalian cellsDepositorAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin
Plasmid#182305PurposeE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.DepositorInsertpH-Countdown insertion into GECO 1.2 calcium sensing domains
TagsA modified TorA periplasmic targeting sequence pl…ExpressionBacterialMutationSpontaneous mutation of calmodulin's penulti…Promotert7Available SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
TagsmCherryExpressionInsectPromoternanosAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RWDD2B_pLENTI-CAG-IRES-GFP
Plasmid#177001PurposeMammalian lentiviral expression vector encoding RWDD2BDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
WDR4_pcDNA6.2/EmGFP-Bsd
Plasmid#176984PurposeMammalian expression vector encoding WDR4 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RWDD2B_pcDNA6.2/EmGFP-Bsd
Plasmid#176956PurposeMammalian expression vector encoding RWDD2B and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCBP3_pcDNA6.2/EmGFP-Bsd
Plasmid#176958PurposeMammalian expression vector encoding PCBP3 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
POFUT2_pcDNA6.2/EmGFP-Bsd
Plasmid#176960PurposeMammalian expression vector encoding POFUT2 and EmGFP-BsdDepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)
Plasmid#174641PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-mVenus-ppKin14VIb(861-1321)
TagsSSPB(micro)-VenusExpressionMammalianMutationSSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…PromoterChicken beta-actinAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-MGW-Cterm_H
Plasmid#146484PurposeInsect Expression of DmGW182-MGW-CtermDepositorInsertDmGW182-MGW-Cterm (gw Fly)
ExpressionInsectAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat
Plasmid#178871PurposeMammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HADepositorInsertPB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3_A
Plasmid#145895PurposeInsect Expression of DmNot3DepositorInsertDmNot3 (Not3 Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence giv…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only