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pDONR223_KRAS_p.G12S

Plasmid

#81654

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.W131G

Plasmid

#81632

Purpose

Gateway Donor vector containing SPOP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway Cloning

Mutation

W131G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.D194Y

Plasmid

#81401

Purpose

Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway Cloning

Mutation

D194Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61K

Plasmid

#81319

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

Q61K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MET_WT

Plasmid

#82307

Purpose

Gateway Donor vector containing MET , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MET (MET Human)

Use

Gateway Cloning

Mutation

S705N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD3_WT

Plasmid

#82272

Purpose

Gateway Donor vector containing SMAD3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD3 (SMAD3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1AN_WT

Plasmid

#82256

Purpose

Gateway Donor vector containing HIF1AN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1AN (HIF1AN Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETV1_WT

Plasmid

#82209

Purpose

Gateway Donor vector containing ETV1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETV1 (ETV1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_CDK4_p.R24C

Plasmid

#82175

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway Cloning

Mutation

R24C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NANOG_WT

Plasmid

#82148

Purpose

Gateway Donor vector containing NANOG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NANOG (NANOG Human)

Use

Gateway Cloning

Mutation

K82N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#82113

Purpose

Gateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERN1_WT

Plasmid

#82115

Purpose

Gateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERN1 (ERN1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway Cloning

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-sgAAVS1

Plasmid

#83906

Purpose

stable knockout

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-neuro-seeERv1.0

Plasmid

#254751

Purpose

Original bifunctional estrogen and CRE reporter

Depositor

J Edward van Veen

Publication

Cara et al Neuron. 2026 Aug 28:S0896-6273(26)00609-

Insert

Estrogen Response Element

Use

AAV

Tags

d2EGFP

Expression

Mammalian

Available since

Aug. 7, 2026

Availability

Academic Institutions and Nonprofits only

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AAV Syn-coca-5HT3-IRES-mCherry

Plasmid

#242197

Purpose

AAV vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Publication

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-coca-5HT3-IRES-mCherry (Htr3a Mouse)

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

Synapsin

Available since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L3-3xFLAG

Plasmid

#201642

Purpose

Over-expression of 3xFLAG-tagged human LUC7L3

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L3 (LUC7L3 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

July 31, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hADAR1_2g)-PGKpuro2ABFP-W

Plasmid

#208434

Purpose

Lentiviral gRNA plasmid targeting human ADAR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Publication

Yee et al Mol Carcinog. 2025 Jun;64(6):1066-1077.

Insert

ADAR1 (ADAR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U8

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-2xFLAG-2xSTREP-E2F1

Plasmid

#236433

Purpose

doxycycline inducible expression of E2F1 in mammalian cells

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-2xFLAG-2xSTREP-E2F1

Plasmid

#236436

Purpose

stable expression of E2F1 using virus infection

Depositor

Michele Pagano

Publication

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E2F1 (E2F1 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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