We narrowed to 7,531 results for: Ski

1. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO.Nfia.Blast

   Plasmid

   #117270

   Purpose

   Expresses Nfia and blasticidin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEND-349_Cacnes_ori

   Plasmid

   #225604

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-DEST47-SIRT1

   Plasmid

   #242131

   Purpose

   Expression in mammalian cells

   Depositor

   J. Carl Barrett, Izumi Horikawa

   Article

   Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

   Insert

   SIRT1 (SIRT1 Human)
   

   Tags

   Cycle 3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. sUPRa

   Plasmid

   #223242

   Purpose

   sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

   Depositor

   Colin Akerman

   Article

   Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

   Insert

   sUPRa
   

   Use

   AAV

   Promoter

   Custom

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-PC-GCaMP6f

   Plasmid

   #73503

   Purpose

   GCaMP6f knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   GCaMP6f
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-Mfn2

   Plasmid

   #141156

   Purpose

   Mammalian expression and labeling mitofusin 2

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   Mitofusin 2 (MFN2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GfaABC1D-DIO-mNeonGreen-mScarlet-l-WPRE

    Plasmid

    #223668

    Purpose

    Cre-dependently expresses mNeonGreen, mScarlet-I in astrocytes

    Depositor

    Jun Nagai

    Article

    Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

    Insert

    mNeonGreen-mScarlet-l
    

    Use

    AAV and Cre/Lox

    Promoter

    GfaABC1D

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. T-2A-EGFP-PGK-Puro

    Plasmid

    #83344

    Purpose

    Donor vector to knocin EGFP into human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    Human Brachyury knockin homology arms (TBXT Human)
    

    Tags

    2A-EGFP

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203228

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. myc-KIF5B

    Plasmid

    #127617

    Purpose

    N-terminal myc-tagged mouse KIF5B

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. T-STOP sgRNA-1

    Plasmid

    #83346

    Purpose

    The sgRNA at the stop codon of human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    T-STOP sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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