We narrowed to 6,927 results for: 11

1. pMedium-FT-N1

   Plasmid

   #31911

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Medium-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-2(KW)2-mCherry

   Plasmid

   #112738

   Purpose

   Express 2(KW)2-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIC-EcTOP

   Plasmid

   #224463

   Purpose

   Expression of recombinant Escherichia coli topoisomerase I

   Depositor

   Yuk-Ching Tse-Dinh

   Article

   Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

   Insert

   Escherichia coli TopA
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP2463

   Plasmid

   #107774

   Purpose

   Expression of EGFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EGFP in pBBR1-mcs5
   

   Use

   Tags

   pLacZ-EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   pLacZ

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTargeT gE

   Plasmid

   #60845

   Purpose

   Expression of the varicella zoster glycoprotein (gE) in mammalian cells

   Depositor

   Charles Grose

   Article

   Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

   Insert

   ORF68 (ORF68 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-Nck1

   Plasmid

   #45903

   Depositor

   Louise Larose

   Article

   Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

   Insert

   Nck-1 (NCK1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-Cas9

   Plasmid

   #89995

   Purpose

   Expresses high levels of WT Cas9 for genome editing

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

   Insert

   hCas9
   

   Use

   Tags

   3xFlag, SV40 NLS, and T2A peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSUB11

    Plasmid

    #169699

    Purpose

    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    3xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHA1.1-Rap2a-V5-Hygro

    Plasmid

    #195510

    Purpose

    lentiviral vector expressing RAP2A CDS with hygromycin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Hygromycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CibN

    Plasmid

    #209671

    Purpose

    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Split-TurboID-N and pmEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-GFAP104-melanopsin-mCherry

    Plasmid

    #122630

    Purpose

    AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

    Depositor

    Edward Boyden

    Article

    Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

    Insert

    melanopsin-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP104

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVC

    Plasmid

    #175063

    Purpose

    expresses mVenus and mCherry pair in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    mVenus + mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pS3

    Plasmid

    #50596

    Purpose

    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Inserts

    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-U6-sgRNA-CMV-puro

    Plasmid

    #169450

    Purpose

    backbone for sgRNA expression

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL-c5x_UnaG

    Plasmid

    #163125

    Purpose

    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    UnaG
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTM1_VP35_ZEBOV

    Plasmid

    #68121

    Purpose

    Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP35 ZEBOV (VP35 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMBA333

    Plasmid

    #214745

    Purpose

    Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 iTEV

    Plasmid

    #64277

    Purpose

    Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent tobacco etch virus protease sensor (iTEV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTM1_VP30_ZEBOV

    Plasmid

    #69119

    Purpose

    Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP30 ZEBOV (VP30 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT222

    Plasmid

    #40942

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. RNF146-c005

    Plasmid

    #173091

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    RNF146 (RNF146 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    R99-L183, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2,0_3E5E_luciferase

    Plasmid

    #69358

    Purpose

    Encodes for Ebola Minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    3E5E luciferase minigenome
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-CaM-NanoLuc-15aa-uTEVp

    Plasmid

    #163034

    Purpose

    FLiCRE protease with luciferin-gating for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP-CaM-NanoLuc-15aa-uTEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF6 VPS52-V5-6His

    Plasmid

    #175096

    Purpose

    V5 tagged VPS52 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    VPS52 (VPS52 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    N666D- please see depositor comment

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tagSrc-HaloTag

    Plasmid

    #179141

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after N534 of Src.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GDAP2-c005

    Plasmid

    #173110

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GDAP2 (GDAP2 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    M1-N231

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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