We narrowed to 16,891 results for: Por
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Plasmid#35240DepositorInsertZinc finger array targeting zgc:65890 (zgc:65890 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
VDAC2_R (OZ524)
Plasmid#27199DepositorInsertZinc finger array targeting VDAC2 (vdac2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
REST corepressor 2_L (OZ553)
Plasmid#28078DepositorInsertZinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
REST corepressor 2_R (OZ554)
Plasmid#28079DepositorInsertZinc finger array targeting REST corepressor 2 (rcor2 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-ITGB1(no stop)
Plasmid#215453PurposeGateway entry vector with integrin beta1 wild-type (WT) without a stop codonDepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorTagsExpressionMutationSilent mutations added to disrupt shRNA binding a…PromoternoneAvailable SinceSept. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF.DEST51-PTPN11(D425A,C459S)-Clover
Plasmid#215526PurposeExpression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)DepositorInsertPTPN11 (PTPN11 Human)
UseTagsCloverExpressionMammalianMutationD425A, C459S (Phosphatase inactive)PromoterEF1aAvailable SinceSept. 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR2b-PTPN11(D425A,C459S)-Clover
Plasmid#215520PurposeGateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)DepositorInsertPTPN11 (PTPN11 Human)
UseGateway entry vectorTagsCloverExpressionMutationD425A, C459S (Phosphatase inactive)PromoternoneAvailable SinceSept. 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235301PurposeComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2-bGH
Plasmid#235300PurposeComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235302PurposeComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-ITGB1(YYFF)
Plasmid#215451PurposeGateway entry vector with integrin beta1 NPxY mutant (YYFF)DepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorTagsExpressionMutationMutations in the NPxY sites Y783F, Y795F; Silent …PromoternoneAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR2b-ITGB1(WT)-mRuby2
Plasmid#215446PurposeGateway entry vector with integrin beta1 tagged with mRuby2DepositorInsertITGB1 (ITGB1 Human)
UseGateway entry vectorTagsmRuby2ExpressionMutationSilent mutations added to disrupt shRNA binding a…PromoternoneAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV hSyn INTRON HaloTag-STIM1 WPRE
Plasmid#236233PurposeAAV expression of mouse STIM1 internally tagged with HaloTagDepositorInsertHaloTag-Stim1 (Stim1 Mouse)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-STIM1 WPRE
Plasmid#236234PurposeAAV expression of mouse STIM1 internally tagged with mEmeraldDepositorInsertmEmerald-Stim1 (Stim1 Mouse)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC84_HBB(HBA1reg-HBA1)
Plasmid#232410PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC83_HBB(HBBreg-HBA1)
Plasmid#232409PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-P577S-V5/HIS
Plasmid#234760PurposeExpression of the P577S mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationP577S substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-R487L-V5/HIS
Plasmid#234761PurposeExpression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal TumorDepositorInsertPDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationR487L substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-G853D-V5/HIS
Plasmid#234757PurposeExpression of the G853D mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-G853D receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationG853D substitutionPromoterCMVAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-T674I-V5/HIS
Plasmid#234756PurposeExpression of the T674I mutant variant of human PDGFRA, which is associated with Idiopathic Hypereosinophilic Syndrome, resistant to imatinibDepositorInsertPDGFRA-T674I receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationT674I substitutionPromoterCMVAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Glu
Plasmid#218458PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Glu substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationThr1273Glu substitutionPromoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-His1271Lys
Plasmid#218456PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (His1271Lys substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationHis1271Lys substitutionPromoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-SARS-CoV-2-S-tail-Lys1269Ala-His1271Ala
Plasmid#218459PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Lys1269Ala and His1271Ala substitutions) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationLys1269Ala and His1271Ala substitutionsPromoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-K627M-V5/HIS
Plasmid#234755PurposeExpression of the K627M kinase defective mutant variant of human PDGFRADepositorInsertPDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationK627M substitutionPromoterCMVAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
MPC1-QQ-FLAG
Plasmid#228905PurposeExpression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tagDepositorInsertMPC1 (Mpc1 Mouse)
UseTagsFLAGExpressionMammalianMutationK45 and K46 motif sites were both replaced with g…PromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9
Plasmid#223323PurposeHuman codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).DepositorInsertSpCas9 and sgRNA-T2 (cas9 )
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)
Plasmid#224496PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-iRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-0K9Q
Plasmid#221743PurposeStable expression of GFP-0K9Q in mammalian cells to monitor membrane chargeDepositorInsertC -terminal region of KRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170, mutated K to QPromoterAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-1K8Q
Plasmid#221742PurposeStable expression of GFP-1K8Q in mammalian cells to monitor membrane chargeDepositorInsertC -terminal region of KRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170, mutated K to QPromoterAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-3K6Q
Plasmid#221741PurposeStable expression of GFP-3K6Q in mammalian cells to monitor membrane chargeDepositorInsertC -terminal region of KRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170, mutated K to QPromoterAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-5K4Q
Plasmid#221740PurposeStable expression of GFP-5K4Q in mammalian cells to monitor membrane chargeDepositorInsertC -terminal region of KRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170, mutated K to QPromoterAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Embigin
Plasmid#221417PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(R76A)
Plasmid#198570PurposeSPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged arginine R76 to alanine (R76A)PromoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast GFP-SPNS1(E164K)
Plasmid#198572PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)PromoterAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu
Plasmid#218454PurposeBacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions (S SARS-CoV-2)
UseTagseXactExpressionBacterialMutationPromoterAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp
Plasmid#218457PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationThr1273Asp substitutionPromoterAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-wt
Plasmid#184269PurposeExpresses shRNA resistant GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationchanged nucleotides 723-747, 1488-1509, 1524-1548…PromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine
Plasmid#202058PurposeInducible AAV with reporter gene and cloning site to add activator domainsDepositorInsertAAV-Rapamycin Inducible-noActivator Domain -Citrine
UseAAV; Aav with reporterTagsExpressionMammalianMutationITRs intactPromoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1L
Plasmid#215043PurposeMammalian expression of human TWIST1 (with fragment of loop from NEUROD1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationLeft portion of TWIST1 bHLH loop (aa137-139, TLP)…PromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_NEUROD1R
Plasmid#215044PurposeMammalian expression of human TWIST1 (with fragment of loop from NEUROD1)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationRight portion of TWIST1 bHLH loop (aa139-141, PSD…PromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HaloTag-KRas4B(CT)
Plasmid#214276PurposeMammalian expression of HaloTag fused to the C-terminal tail of KRas4BDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-E993A
Plasmid#203698Purposeexpresses ATP10B with the pathogenic mutation E993ADepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-E210A
Plasmid#203697Purposeexpresses ATP10B with the catalytic mutation E210ADepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-D433N
Plasmid#203696Purposeexpresses ATP10B with the catalytic mutation D433NDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-V748L
Plasmid#203702Purposeexpresses ATP10B with the pathogenic mutation V748LDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-I1038T
Plasmid#203700Purposeexpresses ATP10B with the pathogenic mutation I1038TDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-R153X
Plasmid#203701Purposeexpresses ATP10B with the pathogenic mutation R153XDepositorAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.3
Plasmid#206137PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pb-miRE-tre-3xFLAG-hnRNPU∆RGG
Plasmid#196088PurposeExpresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cellsDepositorInsertSAF-A (Hnrnpu Mouse)
UseTagsN-terminal 3xFLAGExpressionBacterial and MammalianMutationremoved last 154 amino acids on cterminal endPromoterTREAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd1-NLS-3XFLAG-V5
Plasmid#196092PurposeExpresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 96-285 (predicted disordered …PromoterCAGGSAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only