We narrowed to 22,536 results for: CRISPR

1. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GluN2B (px330:2-guideCas9)

   Plasmid

   #169430

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHS212

   Plasmid

   #87346

   Purpose

   Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S

   Promoter

   T7

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL3287

   Plasmid

   #139750

   Purpose

   Base Cas12a CRISPRi plasmid

   Depositor

   Himadri Pakrasi

   Article

   Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti SpBsmBI sgRNA Puro

   Plasmid

   #62207

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKm-HCBE

   Plasmid

   #170333

   Purpose

   a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized CBEs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9n-D10A

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpyB

   Plasmid

   #220186

   Purpose

   sgRNA expression vector - pBG35 promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG35 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBG35

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pMiniT:GFP-Gephyrin TKIT donor

   Plasmid

   #169433

   Purpose

   Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. rGluA1 (px330:2-guideCas9)

   Plasmid

   #169437

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKm-HGBE

    Plasmid

    #170334

    Purpose

    a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized GBEs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9n-D10A

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Gephyrin (px330:2-guideCas9)

    Plasmid

    #169432

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. GluA1 (px330:2-guideCas9)

    Plasmid

    #169417

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHelper_AcCAST

    Plasmid

    #127923

    Purpose

    Expresses AcCAST and a crRNA targeting PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIF4

    Plasmid

    #196069

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MoRP27 promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

    Plasmid

    #71485

    Purpose

    Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Himar-dCas9

    Plasmid

    #137079

    Purpose

    Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. bRA77

    Plasmid

    #100953

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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