We narrowed to 5,898 results for: dna plasmids

1. LT2_32_PyruEth_X2site

   Plasmid

   #194428

   Purpose

   Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   PyruEth
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Mutation

   NA

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LT1_75_Donor_Tag_RPL13A-mTurq2

   Plasmid

   #194415

   Purpose

   Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   mTurquoise2 CDS
   

   Use

   Synthetic Biology; Used as donor dna in genome in…

   Expression

   Yeast

   Mutation

   NA

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHC_DYOLacI-R

   Plasmid

   #102922

   Purpose

   pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI

   Depositor

   George Church

   Article

   Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

   Insert

   sfGFP
   

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP569

   Plasmid

   #139499

   Purpose

   Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xPP7sl STL1 100 - 600
   

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript alpha-SSeCKS

   Plasmid

   #61295

   Purpose

   contains rat alpha SSeCKS cDNA

   Depositor

   Irwin Gelman

   Insert

   alpha SSeCKS (Akap12 Rat)
   

   Expression

   Bacterial

   Mutation

   cDNA with 1kb of 3' UTR and 150bp of 5'…

   Promoter

   T7

   Available Since

   Dec. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. POLQA

   Plasmid

   #74645

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   POLQA (POLQ Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Promoter

   PH

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-Clover-HIF-1alpha Wildtype

   Plasmid

   #163365

   Purpose

   Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha (HIF1A Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV Promoter

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIN40C.SFFV.MCS.IRES.GFP

   Plasmid

   #169280

   Purpose

   Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIN40C.SFFV.MCS.IRES.dTomato

    Plasmid

    #169279

    Purpose

    Constitutive overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOME240P

    Plasmid

    #182602

    Purpose

    T15

    Depositor

    Dirk Dittmer

    Insert

    T15
    

    Expression

    Mammalian

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMflT-o2

    Plasmid

    #101310

    Purpose

    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mBeta4/pCNA3.1

    Plasmid

    #197316

    Purpose

    Plasmid expressing an antigen used to generate an antibody that targets the BK beta4 potassium channel subunit

    Depositor

    Robert Brenner

    Insert

    Slo B4 (Kcnmb4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgLAP1

    Plasmid

    #19702

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Tags

    EGFP, S peptide, and TEV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgLAP2

    Plasmid

    #19703

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Tags

    Flag, S peptide, and TEV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169283

    Purpose

    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid

    #135668

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgCR8
    

    Use

    Mouse Targeting

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid

    #135669

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgPten
    

    Use

    Mouse Targeting

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEFL

    Plasmid

    #38121

    Depositor

    JS Gutkind

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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