We narrowed to 13,996 results for: eng
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Plasmid#133920PurposeminiICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoterDepositorInsertminiICE-tet(M)cat(PC194)_PT7-GFP
UseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW313lux-hrpR
Plasmid#61436PurposepBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))DepositorInsertF2620-rbs33-hrpR-ter
ExpressionBacterialPromoterpluxAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G-EsaI
Plasmid#47662PurposeesaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)DepositorInsertesaI-term
UseSynthetic BiologyExpressionBacterialPromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6_HEK3+1T>A_(PP7-C4-Q1)
Plasmid#232437PurposepegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locusDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRPromoterU6Available SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pCAG-donor(R2Tg)-GFP(intron)
Plasmid#223248PurposeMammalian expression of the R2Tg RNA donorDepositorInsertR2Tg donor
ExpressionMammalianAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backbonePromoterU6Available SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
hNFAT2-409:TALE:VP64:KRɸ
Plasmid#160661PurposeConstitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.DepositorInserthNFAT2-409:TALE:VP64:KRɸ
TagsMyc tagExpressionMammalianPromoterCMVAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIDFA-CMV-NmScarlet-mAID
Plasmid#140616PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1 mutated F74A
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
ExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-tetoff-mVenus-PICdc42
Plasmid#91876PurposePI(WT): Photo inhibitable (blue light sensitive)DepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPS-7942
Plasmid#80459PurposeIPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942DepositorInsertSucrose phosphate synthase
ExpressionBacterialAvailable SinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN43
Plasmid#61346PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS11 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-ABE8e
Plasmid#206882PurposeExpresses FLAG-tagged ABE8e fused to Gag through a linker sequenceDepositorInsertABE8e
UseCRISPR and LentiviralTagsFLAGPromoterCMVAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorInsertGagZip-MCP
ExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.18_PB-SDC4-PGK-Blast
Plasmid#205563PurposeExpress human SDC4DepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK1008 (pSL7)
Plasmid#191003PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_HaloTag9
Plasmid#175537PurposeStrict cytosolic expression of HaloTag9 in mammalian cellsDepositorInsertNES-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only