We narrowed to 6,078 results for: nls

1. sABE v3.22_N

   Plasmid

   #209553

   Purpose

   pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   TadA-8e(2-76)-2xFRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-I-SceI-Trex2

   Plasmid

   #44024

   Depositor

   Jeremy Stark

   Article

   Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

   Insert

   I-SceI fused to mTREX2
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ-176

   Plasmid

   #195424

   Purpose

   Transient, constitutive expression of synZiFTR: ZF1-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

   Plasmid

   #158742

   Purpose

   Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Neuraminidase A/NL/354/16 H3N2
   

   Use

   Tags

   TwinStrep_sfGFP_tetrabrachion

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT03

   Plasmid

   #223375

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMZ-347

   Plasmid

   #195458

   Purpose

   Lentiviral Integratable, constitutive expression of synZiFTR: ZF10-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pSFFV - 3XFLAG - NLS - ZF1 - p65
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Cas9-162A

   Plasmid

   #84918

   Purpose

   for in vitro transcription of Cas9 with polyA tail encoded

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Cas9
   

   Use

   Tags

   Flag, NLS, and polyA tail

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE2-VRER

    Plasmid

    #159984

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-VRER
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMpGWB116

    Plasmid

    #68570

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Nuclear localization signal-tagged tdTomato (tdTo…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT9748-dCBE

    Plasmid

    #163008

    Purpose

    plasmid expressing CBE with dCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    dCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT222

    Plasmid

    #40942

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Expression

    Mutation

    Promoter

    FCP and U6

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM122: pAAV-EFS-CasRx

    Plasmid

    #166870

    Purpose

    AAV vector for expressing CasRx

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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