Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,447 results for: nls

Clear keywords
Showing: 3821 - 3840 of 6447 results

  1. P2881

    Plasmid
    #248831
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248831-plasmid-map-sequence-id-497164

    Enlarge

  2. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid
    #117274
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    Tags
    mScarlet-i
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV and IRES
    Available Since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    117274-plasmid-map-sequence-id-233028

    Enlarge

  3. SpCas9-miniCGBE1

    Plasmid
    #198548
    Purpose
    Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198548-plasmid-map-sequence-id-388891

    Enlarge

  4. GB1531

    Plasmid
    #215242
    Purpose
    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    PNos:PhiC31:TNos
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215242-plasmid-map-sequence-id-436558

    Enlarge

  5. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

    Enlarge

  6. Sniper2P

    Plasmid
    #193857
    Purpose
    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193857-plasmid-map-sequence-id-382456

    Enlarge

  10. pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1

    Plasmid
    #250908
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Zebrafish, Human)
    Tags
    EGFP on LMNB1 and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250908-plasmid-map-sequence-id-498469

    Enlarge

  17. pTL51_cHS4-CAG-s36GFPHaloTag-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4

    Plasmid
    #223542
    Purpose
    PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells
    Depositor
    Sally Lowell
    Article
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-HaloTag, mCherry-3NLS, PAC
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223542-plasmid-map-sequence-id-450063

    Enlarge

  19. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240513-plasmid-map-sequence-id-477190

    Enlarge

Showing: 3821 - 3840 of 6447 results