We narrowed to 52,923 results for: plasmid

1. pAc5.1B-lambdaN-HA-DmUPF3_1-121-V5His6_N

   Plasmid

   #147005

   Purpose

   Insect Expression of DmUPF3_1-121

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3_1-121 (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   2 silent mutations compared to the sequence given…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM-41-HsNot2_N

   Plasmid

   #147010

   Purpose

   Bacterial Expression of HsNot2

   Depositor

   Elisa Izaurralde

   Insert

   HsNot2 (CNOT2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnEA-NvG-DmNot1_1147-1717_N

   Plasmid

   #147018

   Purpose

   Bacterial Expression of DmNot1_1147-1717

   Depositor

   Elisa Izaurralde

   Insert

   DmNot1_1147-1717 (Not1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmGW182-12xmut_1-539_O

   Plasmid

   #147067

   Purpose

   Insect Expression of DmGW182-12xmut_1-539

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182-12xmut_1-539 (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-EGFP-DmGW182_1-539-dsRNAres_N

   Plasmid

   #146970

   Purpose

   Insect Expression of DmGW182_1-539-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-539-dsRNAres (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-EGFP-DmGW182_1-605-dsRNAres_N

   Plasmid

   #146971

   Purpose

   Insect Expression of DmGW182_1-605-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-605-dsRNAres (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLNHA-C1-HsNot6_165-557_K

   Plasmid

   #146733

   Purpose

   Mammalian Expression of HsNot6_165-557

   Depositor

   Elisa Izaurralde

   Insert

   HsNot6_165-557 (CNOT6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT-NTAP-DmMe31B-mut1_L

   Plasmid

   #146818

   Purpose

   Insect Expression of DmMe31B-mut1

   Depositor

   Elisa Izaurralde

   Insert

   DmMe31B-mut1 (me31B Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIneo-lambdaN-HA-HsTNRC6Biso1_1-1618_H

   Plasmid

   #146442

   Purpose

   Mammalian Expression of HsTNRC6Biso1_1-1618

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6Biso1_1-1618 (TNRC6B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIneo-lambdaN-HA-HsTNRC6Biso1-delMID2_H

    Plasmid

    #146443

    Purpose

    Mammalian Expression of HsTNRC6Biso1-delMID2

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6Biso1-delMID2 (TNRC6B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIneo-lambdaN-HA-HsSmg7_1-632_I

    Plasmid

    #146532

    Purpose

    Mammalian Expression of HsSmg7_1-632

    Depositor

    Elisa Izaurralde

    Insert

    HsSmg7_1-632 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three silent mutation G216A, T1185C and A1521G co…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-60-NusA-DmGW182_1-539_G

    Plasmid

    #146380

    Purpose

    Bacterial Expression of DmGW182_1-539

    Depositor

    Elisa Izaurralde

    Insert

    DmGW182_1-539 (gw Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-60-NusA-HsTNRC6B-UBA_F

    Plasmid

    #146268

    Purpose

    Bacterial Expression of HsTNRC6B-UBA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-UBA (TNRC6B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-Dm4EHP-A_G

    Plasmid

    #146346

    Purpose

    Insect Expression of Dm4EHP-A

    Depositor

    Elisa Izaurralde

    Insert

    Dm4EHP-A (4EHP Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence from …

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-DmAdhV5-5BoxB_X

    Plasmid

    #147910

    Purpose

    Insect Expression of DmAdh-5BoxB

    Depositor

    Elisa Izaurralde

    Insert

    DmAdh-5BoxB (Adh Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. V5-WT-long-UBASH3A

    Plasmid

    #192087

    Purpose

    Mammalian expression of human wild-type long-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V5-W279A-long-UBASH3A

    Plasmid

    #192085

    Purpose

    Mammalian expression of human W279A long-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    W279A

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. V5-K202R-long-UBASH3A

    Plasmid

    #192083

    Purpose

    Mammalian expression of human K202R long-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    K202R

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-W279A-long-UBASH3A-Ntag

    Plasmid

    #192095

    Purpose

    Human W279A long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W279A

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsAGO3-P591GA621FK661AL695Y_AH

    Plasmid

    #148861

    Purpose

    Mammalian Expression of HsAGO3-P591GA621FK661AL695Y

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO3-P591GA621FK661AL695Y (AGO3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SGEP-sh-Hs-PHGDH-67

    Plasmid

    #188671

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SGEP-sh-Hs-PHGDH-1968

    Plasmid

    #188673

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SGEP-sh-Hs-PHGDH-1957

    Plasmid

    #188674

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ipUSEPR-sg-Hs-PHGDH-2

    Plasmid

    #188676

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SGEP-sh-Hs-PHGDH-1967

    Plasmid

    #188672

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ipUSEPR-sg-Hs-PHGDH-5

    Plasmid

    #188679

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ipUSEPR-sg-Hs-PHGDH-6

    Plasmid

    #188680

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ipUSEPR-sg-Hs-PHGDH-4

    Plasmid

    #188678

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ipUSEPR-sg-Hs-PHGDH-3

    Plasmid

    #188677

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU-YLL058W-zr

    Plasmid

    #176565

    Purpose

    Expression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Insert

    YLL058W-zr
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimised for expression in S.cerevisae

    Promoter

    TDH1(GAP)

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAc5.1B-myc-SBP-DmDCP2-dsRNAres_J

    Plasmid

    #146629

    Purpose

    Insect Expression of DmDCP2-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2-dsRNAres (DCP2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSFDuetG-DmDCP1_1-127-DmXRN1_1312-1383_N

    Plasmid

    #147021

    Purpose

    Bacterial Expression of DmDCP1_1-127-DmXRN1_1312-1383

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP1_1-127-DmXRN1_1312-1383 (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAc5.1B-EGFP-DmDCP2-PC-V5His6_G

    Plasmid

    #146348

    Purpose

    Insect Expression of DmDCP2-PC

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2-PC (DCP2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1B-lambdaN-HA-DmDCP2-PC-V5His6_G

    Plasmid

    #146360

    Purpose

    Insect Expression of DmDCP2-PC

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2-PC (DCP2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. KBB258

    Plasmid

    #185072

    Purpose

    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion

    Depositor

    Ken Belanger

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Yeast

    Mutation

    Nup1 N-term+FXFG repeats

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. KBB611

    Plasmid

    #185112

    Purpose

    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification

    Depositor

    Ken Belanger

    Insert

    POM152
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Pom152 repeats 4&5-6His

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p416-GPD-DIPP3B-HA

    Plasmid

    #183949

    Purpose

    Expresses human HA-tagged DIPP3B from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    DIPP3B (NUDT11 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    WT

    Promoter

    GPD

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT7-EGFP-C1-HsDCP1b_N

    Plasmid

    #147022

    Purpose

    Mammalian Expression of HsDCP1b

    Depositor

    Elisa Izaurralde

    Insert

    HsDCP1b (DCP1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    inserted Q555

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pnEA-NpM-DmNot11_Q

    Plasmid

    #147307

    Purpose

    Bacterial Expression of HsNot11

    Depositor

    Elisa Izaurralde

    Insert

    HsNot11 (CNOT11 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAc5.1B-lambdaN-HA-DmPasilla_B

    Plasmid

    #145943

    Purpose

    Insect Expression of DmPasilla

    Depositor

    Elisa Izaurralde

    Insert

    DmPasilla (ps Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAc5.1B-lambdaN-HA-DmAUB_A

    Plasmid

    #145851

    Purpose

    Insect Expression of DmAUB

    Depositor

    Elisa Izaurralde

    Insert

    DmAUB (aub Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAc5.1B-lambdaN-HA-DmDCP2_457-791_A

    Plasmid

    #145854

    Purpose

    Insect Expression of DmDCP2_457-791

    Depositor

    Elisa Izaurralde

    Insert

    DmDCP2_457-791 (DCP2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. thoc5.018.389

    Plasmid

    #137266

    Purpose

    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

    Depositor

    Tara Davis

    Insert

    thoc5 (THOC5 Human)
    

    Use

    Tags

    (His)6-TEV

    Expression

    Bacterial

    Mutation

    018-389

    Promoter

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. NKAP.001.140

    Plasmid

    #137206

    Purpose

    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

    Depositor

    Tara Davis

    Insert

    NKAP (NKAP Human)
    

    Use

    Tags

    (His)6-TEV

    Expression

    Bacterial

    Mutation

    001-140

    Promoter

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Nup54-mEGFP494(2)

    Plasmid

    #163428

    Purpose

    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP

    Depositor

    Sanford Simon

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGEX4-4T-1 Pontibacillus AGSC

    Plasmid

    #161814

    Purpose

    Expresses AGSC domain from Pontibacillus A/G cyclase enzyme

    Depositor

    Douglas Feldman

    Insert

    AGSC domain
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLIX403-EBFP2-ccdB-EYFP

    Plasmid

    #158558

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pXZ13lacZalphaKanR

    Plasmid

    #160230

    Purpose

    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. RV-Cag-Dio-mVenus

    Plasmid

    #153202

    Purpose

    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the CAG promoter

    Depositor

    Lynette Lim

    Insert

    membrane-tagged-Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. YARS2_pNIC-CTB10H

    Plasmid

    #153060

    Purpose

    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2

    Depositor

    Susanne Gräslund

    Insert

    YARS2 (YARS2 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge