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pWZ414-F47

Plasmid

#23071

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Glutamines…

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F49

Plasmid

#23073

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Arginine, …

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F50

Plasmid

#23074

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R…

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F51

Plasmid

#23075

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Glutamine,…

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F52

Plasmid

#23076

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Arginines,…

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F54

Plasmid

#23078

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F36

Plasmid

#23069

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glycine, H3 K14G

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F43

Plasmid

#23070

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pmCherry-C1-FAK-HA-V744G

Plasmid

#35040

Depositor

Anna Huttenlocher

Publication

Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

focal adhesion kinase (Ptk2 Mouse)

Tags

2x HA and mCherry

Expression

Mammalian

Mutation

GFP-FAK-V744G was generated from GFP-FAK using th…

Available since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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MT-AvrXa10-FT

Plasmid

#234373

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

Depositor

Michael Smanski

Publication

Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

Inserts

NbGP41-GFP-AvrXa10-GB1
dCas9-24XGP41
gRNAs targeting FT locus
RFP

Use

Synthetic Biology

Tags

GB1, GFP, and GP41

Expression

Plant

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAGD236

Plasmid

#219497

Purpose

pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

sfGFP, Eco1 msr, Eco1 RT

Expression

Bacterial

Promoter

T7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-uMASS_1

Plasmid

#209762

Purpose

Expression of uMASS_1 in mammalian cells under CAG promoter

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

uMASS_1

Expression

Mammalian

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Publication

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[N150TAA]

Plasmid

#164581

Purpose

superfolder GFP with N150TAA mutation

Depositor

Ryan Mehl

Publication

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-150TAA

Tags

6xHis

Expression

Bacterial

Mutation

N150TAA Mutation

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1 sgTollip

Plasmid

#196546

Purpose

Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

Depositor

David Tumbarello

Publication

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-egfp-U3-UL8

Plasmid

#166694

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Depositor

Yujia Cai

Publication

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)

Use

Lentiviral

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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